Mouse ifn γ elisa kit

The serum was dissolved at room temperature and mixed well. The cell culture supernatant of HaCAT cells after indicated treatment was obtained after centrifugation (1000 r/min, 10 min). The levels of IFN-γ, IL-8 and IL-6 in the mice serum were detected by Mouse IFN-γ ELISA Kit (Beyotime), Mouse IL-8 ELISA Kit (Wuhan Saipei Biotechnology Co., Ltd) and Mouse IL-6 ELISA Kit (Beyotime) and the levels of IFN-γ, IL-8 and IL-6 in the culture supernatant of HaCAT cells were detected by Human ELISA Kits (Beyotime).

The intratumour or serum value of cytokines and proteins was determined using ELISA experiments according to the manufacturers’ protocols (FastScan™ Total PD-L1 ELISA Kit Mouse–Preferred, Cell Signalling Technology, Cat. #41590; Mouse FLT3 ligand ELISA kit, Abcam, Cat. #ab275551; Mouse sCD40 ligand ELISA Kit, WESTANG, Cat. #F00411; Mouse TNF-a ELISA Kit, WESTANG, Cat. #F11630; Mouse IFN-γ ELISA Kit, Beyotime, Cat. # PI508; Mouse IL-12 p70 ELISA Kit, Beyotime, Cat. # PI530). To quantify the intratumour cytokines, 100 mg of tumour tissue was homogenised with 500 μl of ice-cold 1× PBS. The mixture was centrifuged (12,000×g for 5 min), and the supernatant was collected for further ELISA experiments. To quantify the serum cytokines, 100 μl of blood was centrifuged (3000×g for 5 min), and the supernatant serum was collected for further ELISA experiments. Serum samples were sent to BIOS Biological Company Shanghai for quantification of serum ALT and AST values.

The levels of tumor necrosis factor α (TNF-α), interferon-gamma (IFN-γ), CXCL10, and CXCL9 in the co-culture system were detected according to the instructions of Human TNF-α ELISA Kit (D711045, Shanghai Sangon Biological Engineering Technology & Services Co., Ltd., Shanghai, China), Human IFN-γ ELISA Kit (D711044, Sangon), Human CXCL10/IP-10 ELISA Kit (PC208, Beyotime), and MIG/CXCL9 Human ELISA Kit (EHCXCL9, Thermo Fisher).
The levels of TNF-α, IFN-γ, CXCL10, and CXCL9 in mouse xenograft tumors were detected according to the instructions of Mouse TNF-α ELISA Kit (PT512, Beyotime), Mouse IFN-γ ELISA Kit (PI508, Beyotime), Mouse CXCL10/IP-10 ELISA Kit (PC206, Beyotime), and MIG/CXCL9 Mouse ELISA Kit (EMCXCL9, Thermo Fisher).