In order to quantify cell growth in the presence of drug, cells (2000 cells in 100 μl of media per well) were plated in flat-bottomed 96-well plates and treated with 4 or 8 doses of a serial dilution of the compound of interest. Each condition was plated in triplicate. After three days, cell proliferation was determined using a WST1 assay (Millipore) according to the manufacturer’s instructions. Absorbance was read at 440 nm and 690 nm using a BioTek Synergy Neo HTS Plate Reader. Data were used to generate dose response curves as described below.
Synergy neo hts plate reader
Manufactured by Agilent Technologies
Sourced in United States
The Synergy Neo HTS Plate Reader is a high-throughput microplate reader designed for various types of assays. It features multi-mode detection capabilities, including absorbance, fluorescence, and luminescence measurements. The core function of the Synergy Neo is to accurately and efficiently quantify samples in a microtiter plate format.
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Lab products found in correlation
3 protocols using synergy neo hts plate reader
1
Cell Proliferation Assay with Drug Treatment
2
Cell Proliferation Assay with Drug Treatment
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In order to quantify cell growth in the presence of drug, cells (2000 cells in 100 μl of media per well) were plated in flat-bottomed 96-well plates and treated with 4 or 8 doses of a serial dilution of the compound of interest. Each condition was plated in triplicate. After three days, cell proliferation was determined using a WST1 assay (Millipore) according to the manufacturer’s instructions. Absorbance was read at 440 nm and 690 nm using a BioTek Synergy Neo HTS Plate Reader. Data were used to generate dose response curves as described below.
3
Optimizing BMSC Proliferation with KRMBTI-Serum
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The effects of the different blood sampling time-points, doses of KRMBT and concentrations of KRMBTI-serum on the proliferation of BMSCs were determined by MTT assay. The P3-generation BMSCs were seeded at 1×104 cells/well, and RMBTI-serum was added after 24 h. Five blood sampling time-points (0.5, 1, 1.5, 2, 2.5 h) and 10 KRMBTI-serum addition concentrations (5, 10, 15, 20, 25, 30, 40, 50, 60 and 80%) were set. Each concentration had three repeated wells, and one blank control well was prepared. The final liquid volume of each well was 200 μl. After 72 h, 20 μl 5 mg/ml MTT (Shanghai Sangon Biological Engineering Technology & Services Co., Ltd., Shanghai, China) was added to each well and the plate was incubated for 4 h. Finally the optical density (OD) value at 450 nm of each well was measured using a Synergy NEO HTS plate reader (BioTek Instruments, VT, USA).
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