Retinol, retinaldehyde and retinoic acid were obtained from Sigma-Aldrich (St. Louis, MO) and were prepared as 1 mM stock in DMSO and stored in the vapor phase of nitrogen liquid. Corexit-EC9500A and Corexit-EC9527A (Nalco Energy Services, L.P., Sugar Land, TX) were kindly provided by Dr. Paddy Wiesenfeld at the U.S. FDA [41 (link)]. SPAN®80 (CAS#1338-43-8), TWEEN®80 (CAS#9005-65-6), TWEEN®85 (CAS#9005-70-3), Dioctyl sulfosuccinate sodium salt (DOSS, CAS#577-11-7), Di-(propylene glycol) butyl ether (CAS#29911-28-2), 2-Butoxyethanol (CAS#111-76-2), and 1,2-Propanediol (CAS#57-55-6) were purchased from Sigma-Aldrich. Chemical concentration of 1ppm is defined as 0.0001% v/v except for DOSS, which is 0.0001% w/v.
2 butoxyethanol
Manufactured by Merck Group
Sourced in Germany, United Kingdom
2-butoxyethanol is an organic chemical compound commonly used in laboratory settings. It is a colorless, viscous liquid with a mild odor. The core function of 2-butoxyethanol is as a solvent and coupling agent for various applications.
Automatically generated - may contain errors
Lab products found in correlation
Retinoic acid, by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
Span 80, by Merck Group (1 mentions)
Retinaldehyde, by Merck Group (1 mentions)
Retinol, by Merck Group (1 mentions)
1 2 propanediol, by Merck Group (1 mentions)
Tween 85, by Merck Group (1 mentions)
Dioctyl sulfosuccinate sodium salt, by Merck Group (1 mentions)
Dv 3 ultra, by Ametek (1 mentions)
4 4 methylene bis 2 chloroaniline, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Merck Group (1 mentions)
Benzoic acid, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Octanal, by Merck Group (1 mentions)
α terpineol, by Merck Group (1 mentions)
Nonanal, by Merck Group (1 mentions)
Vanillin, by Merck Group (1 mentions)
Diethyl phthalate, by Merck Group (1 mentions)
Benzophenone, by Merck Group (1 mentions)
D limonene, by Merck Group (1 mentions)
4 protocols using 2 butoxyethanol
1
Standardized Dispersion Protocol for Oil Spills
2
Hydrophobic Ink Formulation Protocol
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The hydrophobic ink used in this study
was prepared using fumed silica with an average nanoparticle size
of 16 nm, epoxy resin (poly bisphenol A-co-epichlorohydrin,
Sigma-Aldrich, South Korea), reagent (2-butoxyethanol, Sigma-Aldrich,
South Korea), and a curing agent (4,4′-methylene bis (2-chloroaniline),
Sigma-Aldrich, South Korea). Initially, a silica solution was created,
mixing 0.45 g of fumed silica (Aerosoil R 972, Evonik Industry, Germany)
and 9.42 g of 2-butoxyethanol with a magnetic stirrer at room temperature
for 8 h. A separate epoxy resin solution was formed by mixing 0.50
g of epoxy resin pellets, 0.08 g of curing agent, and 9.55 g of 2-butoxyethanol
with a magnetic stirrer at 70 °C for 8 h. Subsequently, the silica
and epoxy resin solutions were combined in an equal volume ratio (50%
by volume) and mixed for an additional 1 h at room temperature. The
resulting solution was then sonicated in an ultrasonic bath for 5
min to ensure a thorough mixture. The formulated solution had a viscosity
of 11 cP and a surface tension of 23.17 mN/m as measured by a viscometer
(DV -III Ultra, Brookfield, USA) at 10 rpm and a surface tension analyzer
(SEO-DST30M, SEO, South Korea), respectively.
was prepared using fumed silica with an average nanoparticle size
of 16 nm, epoxy resin (poly bisphenol A-co-epichlorohydrin,
Sigma-Aldrich, South Korea), reagent (2-butoxyethanol, Sigma-Aldrich,
South Korea), and a curing agent (4,4′-methylene bis (2-chloroaniline),
Sigma-Aldrich, South Korea). Initially, a silica solution was created,
mixing 0.45 g of fumed silica (Aerosoil R 972, Evonik Industry, Germany)
and 9.42 g of 2-butoxyethanol with a magnetic stirrer at room temperature
for 8 h. A separate epoxy resin solution was formed by mixing 0.50
g of epoxy resin pellets, 0.08 g of curing agent, and 9.55 g of 2-butoxyethanol
with a magnetic stirrer at 70 °C for 8 h. Subsequently, the silica
and epoxy resin solutions were combined in an equal volume ratio (50%
by volume) and mixed for an additional 1 h at room temperature. The
resulting solution was then sonicated in an ultrasonic bath for 5
min to ensure a thorough mixture. The formulated solution had a viscosity
of 11 cP and a surface tension of 23.17 mN/m as measured by a viscometer
(DV -III Ultra, Brookfield, USA) at 10 rpm and a surface tension analyzer
(SEO-DST30M, SEO, South Korea), respectively.
3
Comprehensive Chemical Standards Identification
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Acetonitrile HPLC grade, methanol, and hexane GC-MS grade were supplied by Merck (Darmstadt, Germany). Ethanol for analysis was supplied by Merck (Darmstadt, Germany). The 2-butoxyethanol, spectrophotometric grade with a purity of ≥99.0%, 2,2-dimethyl-1,3-propanediol with a purity of 99.0%, octanal with a purity of 99%, D-Limonene, ε-Caprolactam; 2,6-Di-tert-butyl-1,4-benzoquinone with a purity of 98.0%, diethyl phthalate with a purity of 99.5%, benzophenone with a purity of ≥99.0%, benzoic acid with a purity of 99.5%, vanillin with a purity of 99.0%, 2,4-ditertbutylphenol with a purity of 99.0%, and α-terpineol were purchased from Sigma Aldrich (Steinheim, Germany). Nonanal with purity of 98.7% was purchased from Supelco (Bellefonte, PA, USA). The 2-phenoxyethanol with purity of ≥99.0% was supplied by Fluka (Seelze, Germany). Working solutions were prepared by diluting different amounts of the stock standard solution in methanol.
4
Cell Viability Assay with MTT
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MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was used to assess cell viability. HaCaT cells were grown in a 24 well plate for 24 hours, then treated for a further 24 hours with 200nM peptides in media. Peptide was then removed and the tetrazolian MTT dye (Sigma-Aldrich, Poole, UK) added at a concentration of 0.5mg/ml in cell media for 1 hour. Cells were then lysed with 2-butoxyethanol (Sigma-Aldrich, Poole, UK) and the absorbance of supernatants measured at 562nm.
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