Slices of the decellularized lungs and control were prepared for imaging. The samples for SEM were fixed with 2% glutaraldehyde and 2.5% paraformaldehyde in 0.1-M cacodylate buffer (EMD Biosciences, USA) for 2 h at room temperature, rinsed in cacodylate buffer, and dehydrated through an ethanol gradient. The samples were further dehydrated in hexamethyldisilizane for 10 min and dried overnight, sputter-coated with gold, and analyzed by using the scanning electron microscope Hitachi Analytical Table Top Microscope TM3000 (Hitachi, Tokyo, Japan), with 15-kVa acceleration.
Analytical table top microscope tm3000
Manufactured by Hitachi
Sourced in Japan
The Hitachi Analytical Table Top Microscope TM3000 is a compact, user-friendly microscope designed for routine analysis and observation. It features a high-resolution imaging system and is capable of magnifications up to 30,000x. The microscope is equipped with a stable platform and intuitive controls, making it suitable for a wide range of laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using analytical table top microscope tm3000
1
Lung Decellularization and SEM Imaging
2
Scanning Electron Microscopy of Decellularized Lungs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Slices of the decellularized lungs and control were prepared for imaging. The
samples for scanning electron microscopy (SEM) were fixed with 2% glutaraldehyde
and 2.5% paraformaldehyde in 0.1-M cacodylate buffer (EMD Biosciences) for 2 h
at room temperature, rinsed in cacodylate buffer, and dehydrated through an
ethanol gradient. The samples were further dehydrated in hexamethyldisilizane
for 10 min and dried overnight, sputter-coated with gold, and analyzed using the
scanning electron microscope Hitachi Analytical Table Top Microscope TM3000
(Hitachi) with 15-kVa acceleration.
samples for scanning electron microscopy (SEM) were fixed with 2% glutaraldehyde
and 2.5% paraformaldehyde in 0.1-M cacodylate buffer (EMD Biosciences) for 2 h
at room temperature, rinsed in cacodylate buffer, and dehydrated through an
ethanol gradient. The samples were further dehydrated in hexamethyldisilizane
for 10 min and dried overnight, sputter-coated with gold, and analyzed using the
scanning electron microscope Hitachi Analytical Table Top Microscope TM3000
(Hitachi) with 15-kVa acceleration.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!