Western blots to analyse protein expression were conducted using the standard protocol as described previously 17 , 18 . Primary antibodies used were anti‐L‐type Ca2+ channel (Abcam, Cambridge, UK), anti‐ryanodine receptor (Abcam), anti‐SERCA2a (Santa Cruz Biotechnology, Dallas, TX, USA), anti‐NCX (Cell Signaling, Danvers, MA, USA), anti‐phospho‐PLB (Millipore, Bellerica, MA, USA), anti‐total‐PLB (Millipore), anti‐PMCA1 (Abcam), anti‐PMCA4 (Abcam), anti‐RAGE (Abcam), anti‐phospho‐p38 (Cell Signaling), anti‐total‐p38 (Cell Signaling), anti‐iNOS (Abcam), anti‐α‐tubulin (Calbiochem) and anti‐GAPDH (Santa Cruz). For visualization, we used HRP‐conjugated secondary antibodies, which were obtained from either Cell Signaling or Dako.
Anti pmca1
Manufactured by Abcam
Sourced in United States
Anti-PMCA1 is a primary antibody that detects the Plasma Membrane Calcium ATPase 1 (PMCA1) protein. PMCA1 is a calcium pump involved in the regulation of intracellular calcium levels. This antibody can be used for the detection and analysis of PMCA1 in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti serca2a, by Santa Cruz Biotechnology (1 mentions)
Anti gapdh, by Santa Cruz Biotechnology (1 mentions)
Anti total p38, by Cell Signaling Technology (1 mentions)
Anti rage, by Abcam (1 mentions)
Anti phospho p38, by Cell Signaling Technology (1 mentions)
Anti inos, by Abcam (1 mentions)
Gel imager, by Bio-Rad (1 mentions)
Anti grp94, by Abcam (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Anti β actin, by Abcam (1 mentions)
Phosphatase inhibitor cocktail, by Roche (1 mentions)
Anti grp78, by Abcam (1 mentions)
Anti dnp ige, by Merck Group (1 mentions)
Anti nfatc1, by Abcam (1 mentions)
Anti grb2, by Cell Signaling Technology (1 mentions)
3 protocols using anti pmca1
1
Western Blot Analysis of Cardiac Proteins
2
Western Blotting of MCAO Mice Cortex
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The cortical tissues of MCAO mice were dissected for western blotting. Briefly, the protein was extracted by RIPA buffer containing protease inhibitor cocktail, phosphatase inhibitor cocktail, and phenylmethanesulfonyl fluoride (Roche, IN, USA). The concentration of protein was examined using BCA kits. Equal amounts of protein were separated by electrophoresis in 10% sodium dodecyl sulfate polyacrylamide gels and were transferred to nitrocellulose membranes which were incubated with 5% nonfat milk solute that contained 0.1% Tween-20 (TBST) for 2 h. Then, the membranes were incubated with the following primary antibodies at 4°C overnight: anti-CSPG4, anti-KDELR, anti-PMCA1, anti-phospho-Akt and total Akt, anti-phospho-Src and total Src, anti-phospho-p38 and total-p38, anti-phospho-CREB and total-CREB, anti-GRP94, anti-GRP78, and anti-β-actin (Abcam, Cambridge, USA). Membranes were then incubated for 1 h with the secondary antibodies, which were purchased from Abcam (Abcam, Cambridge, USA). These blots were visualized by a gel imager (Bio-Rad, USA).
3
FcεRI Signaling and Lipogenesis Regulation
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