Biochemical tests were carried out using the colorimetric reagent cards GN (gram-negative) and BCL (gram-positive spore-forming bacilli) of the VITEK® 2 Compact 30 system (BioMérieux, Marcy l'Étoile, France) according to the manufacturer’s instructions. The generated data were analyzed using VITEK® 2 software version 7.01, according to the manufacturer's instructions.
Vitek 2 compact 30 system
Manufactured by bioMérieux
Sourced in France
The VITEK® 2 Compact 30 system is a compact, automated instrument for the identification and antimicrobial susceptibility testing of microorganisms. It utilizes fluorescence-based technology to perform rapid and accurate analysis of clinical samples.
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Lab products found in correlation
5 protocols using vitek 2 compact 30 system
1
Biochemical Tests Using VITEK 2 Compact 30
2
Biochemical Analysis of Remediated AMD
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Subsequent to the metagenomics analysis of treated AMD (Sb), Sb was subjected to a series of biochemical reactions in VITEK® 2 Compact 30 system (BioMérieux, France). Cycloheximide supplemented nutrient agar was used for culturable bacteria species from the remediated AMD in plates incubated at 37 °C for 24 h subsequent to sub-culturing for strain purification. Gram reaction, malachite green, and methylene blue staining techniques were used as preliminary identification methods of gram-negative and gram-positive bacteria, including bacterial with endospore, respectively. Further details of the biochemical reactions are as described in [30 (link)].
3
Multidrug-Resistant Acinetobacter baumannii Characterization
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Seventy-five MDR-AB complex isolates were collected from the lower respiratory tract of hospitalized ICU patients between June 2017 and August 2018 and were designated AB-1 to AB-75, based on admission date. The lower respiratory tract specimens were obtained by tracheal aspiration.
Strains were cryopreserved until needed for WGS. Initial identification was conducted using the VITEK2 Compact 30 System (bioMérieux). Antimicrobial susceptibility was determined via microdilution in accordance with guidelines of the Clinical and Laboratory Standards Institute (M100-S29). MDR was defined as resistance to at least one representative agent from three or more of the five antimicrobial-agent classes: cephalosporins (such as ceftazidime or cefepime), carbapenems (such as imipenem), β-lactamase inhibitors (such as cefoperazone/sulbactam), fluoroquinolones (such as ciprofloxacin), and aminoglycosides (such as amikacin) (Magiorakos et al., 2012 (link)).
Strains were cryopreserved until needed for WGS. Initial identification was conducted using the VITEK2 Compact 30 System (bioMérieux). Antimicrobial susceptibility was determined via microdilution in accordance with guidelines of the Clinical and Laboratory Standards Institute (M100-S29). MDR was defined as resistance to at least one representative agent from three or more of the five antimicrobial-agent classes: cephalosporins (such as ceftazidime or cefepime), carbapenems (such as imipenem), β-lactamase inhibitors (such as cefoperazone/sulbactam), fluoroquinolones (such as ciprofloxacin), and aminoglycosides (such as amikacin) (Magiorakos et al., 2012 (link)).
4
Identification of Bacterial Strains via VITEK 2 and Ribotyping
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The 140623 and SBS-1 strains were identified using tests for the VITEK® 2 GP ID card in the VITEK 2 Compact 30 system (BioMérieux SA; http://www.biomerieux.com ). The growth and biochemical characteristics of these strains were assessed by the Bacteria Preservation Center from the Institute of Microbiology, Chinese Academy of Sciences (Beijing, China). The DuPont™ RiboPrinter® System (Hygiena, LLC) was used to identify species by ribotyping (DuPont).
5
Environmental Surveillance for Nosocomial Pathogens
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Research assistants swabbed potential fomites in the patient environment during outbreaks. Cotton-tipped swabs were moistened with sterile saline and surfaces vigorously scrubbed. Potential fomites included ventilator control panel and sensor, bed rails, supply cabinets, computer keyboards and mice, curtains, air vents, door handles, supply carts, and mattresses.
Transtracheal aspiration specimens were inoculated onto blood agar and MacConkey agar and incubated at 37°C for 48 h in an air incubator. Environmental swabs were incubated overnight in fastidious broth and then subcultured onto blood agar and MacConkey agar. Identification was conducted using the VITEK2 Compact 30 System (bioMerieux). The antimicrobial susceptibility was determined by microdilution in accordance with the Clinical and Laboratory Standards Institute guidelines.
Transtracheal aspiration specimens were inoculated onto blood agar and MacConkey agar and incubated at 37°C for 48 h in an air incubator. Environmental swabs were incubated overnight in fastidious broth and then subcultured onto blood agar and MacConkey agar. Identification was conducted using the VITEK2 Compact 30 System (bioMerieux). The antimicrobial susceptibility was determined by microdilution in accordance with the Clinical and Laboratory Standards Institute guidelines.
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