Rabbit anti atg16l1

Manufactured by Abcepta

Sourced in United Kingdom

Rabbit anti-Atg16L1 is a primary antibody that specifically binds to the Atg16L1 protein. Atg16L1 is a key component of the autophagy pathway and is involved in the formation of autophagosomes. This antibody can be used to detect and quantify the Atg16L1 protein in various experimental applications.

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ATG16L1 (2 citations)

2 protocols using rabbit anti atg16l1

Immunofluorescence Staining of Neural Markers

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Primary antibodies used were: mouse anti-BrdU (G3G4; DSHB), mouse anti-GFAP (Thermo Fisher Scientific), rhodamine-conjugated mouse anti-BrdU (Millipore), mouse anti-nestin (Rat-401), mouse anti-NeuN (Millipore), mouse anti–Nqo-1 (Santa Cruz Biotechnology), mouse anti-p62 (Abcam), mouse anti ubiquitin (Santa Cruz Biotechnology), mouse antivinculin (Sigma-Aldrich), rabbit anti-Atg5 (Novus Biologicals), rabbit anti-Atg16L1 (Abgent), rabbit anti-GFAP (Dako), rabbit anti-Ki67 (Spring Bioscience), rabbit anti-LC3 (Cell Signaling), rabbit anti-NBR1 (Cell Signaling), rabbit anti-Nrf2 (Abcam), rabbit anti-p62 (Enzo), rabbit anti-Sox2 (Millipore), rabbit anti-TAX1BP1 (Abgent), rabbit anti-Fip200 (ProteinTech), rabbit anti-olig2 (Millipore), rat anti-Ki67 (BioLegend), and guinea pig antidoublecortin (anti-DCX; EMD Millipore). Secondary antibodies were goat anti–rabbit IgG-FITC, goat anti–rabbit IgG–Texas red, goat anti–mouse IgG-FITC, goat anti–mouse IgG–Texas red, goat anti–mouse IgG-HRP, and goat anti–rabbit IgG-HRP (Jackson Immunology).
DHE and EUK-8 were purchased from Sigma-Aldrich. EUK-134 was purchased from Cayman Chemical. DCFDA, MitoTracker Red, and MitoTracker Green were purchased from Invitrogen.

Wang C., Chen S., Yeo S., Karsli-Uzunbas G., White E., Mizushima N., Virgin H.W, & Guan J.L. (2016). Elevated p62/SQSTM1 determines the fate of autophagy-deficient neural stem cells by increasing superoxide. The Journal of Cell Biology, 212(5), 545-560.

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Western Blot Analysis of Autophagy Markers

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Liver tissues or cultured cells were homogenized as described previously [40 (link)]. Equal amounts of protein were separated on 12 % gels by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinyldifluoride membranes. The membranes were incubated overnight at 4°C with primary antibodies: rabbit anti-HO-1(1:1000, Abcam, Cambridge, UK), rabbit anti-LC3B (1:1000, Abcam), rabbit anti-Atg16L1(1:1000, Abgent, San Diego, CA), rabbit anti-calpain2 (1:500, Cell Signaling Technology, Beverly, MA), rabbit anti-Atg7 (1:500, Cell Signaling Technology) and rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:20000; Sigma-Aldrich). Immunoreactive band signals were developed using ECL system (GE Healthcare, Buckinghamshire, UK) and visualized on the Kodak Image Station (Carestream Health Inc, New York, USA). Relative band densities were quantified using the Image J software (NIH, Bethesda, USA).

Liu A., Guo E., Yang J., Li R., Yang Y., Liu S., Hu J., Jiang X., Dirsch O., Dahmen U., Sun J, & Ouyang M. (2016). Ischemic preconditioning attenuates ischemia/reperfusion injury in rat steatotic liver: role of heme oxygenase-1-mediated autophagy. Oncotarget, 7(48), 78372-78386.

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