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2 protocols using linoleic acid

1

Reagents Used in Multidisciplinary Research

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The following reference chemicals were used in the experiments: butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), resazurin, 2,3,5-triphenyltetrazolium chloride (TTC), chloramphenicol, nystatin, quercetin, pyrogallol Cremophor EL, all provided by Sigma-Aldrich (St. Louis, MO, USA); ascorbic acid (AAc), purchased from Dinâmica (Diadema, São Paulo, Brazil); linoleic acid, obtained from Vetec (Duque de Caxias, Rio de Janeiro, Brazil); 2,2-diphenyl-1-picrylhydrazyl (DPPH), provided by FLUKA (St. Louis, MO, USA); silver sulphadiazine 2% (União Química, Brasília, Distrito Federal, Brasil); paracetamol (Johnson & Johnson, São José dos Campos, São Paulo, Brazil); dipyrone (Sanofi Aventis, São Paulo, Brazil); ketamin (Cristália, Itapira, São Paulo, Brazil); and xylazine (Intervet Schering-Plough, São Paulo, Brazil). Stock solutions of these chemicals were prepared with suitable solvent and dilutions were made fresh on the day of experiment. Mueller-Hinton Agar and Mueller-Hinton Broth culture mediums were both purchased of HiMedia (Mumbai, India) and prepared with sterile distilled water.
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2

Antioxidant Characterization of Phenolic Compounds

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The chemical reagents ABTS ((2, 5, 7, Tween 40, and phenolic compound standards (gallic acid, catechin, chlorogenic acid, caffeic acid, ferulic acid, vanillin, quercetin, were obtained from Sigma-Aldrich (Saint Louis, Missouri, USA). methanol (HPLC grade) was obtained from J-TBacker (Alfragide, Lisbon, Portugal). In turn, the Folin-Ciocalteu reagent was obtained from Dinâmica (Indaiatuba, São Paulo, Brazil). Acetone, acetic acid, gallic acid, linoleic acid, methanol, and chloroform were obtained from Vetec (Saint Louis, Missouri, USA), while sulfuric acid was obtained from Merck (Darmstadt, Hessen, Germany). Ethanol, was obtained from Synth (Diadema, São Paulo, Brazil), while n-hexane and potassium persulfate were obtained from Neon (São Paulo, São Paulo, Brazil). All reagents were analytical grade, and stock and buffer solutions were prepared using distilled water.
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