Anti cofilin 1

Manufactured by Abcam

Sourced in China, United Kingdom

Anti-cofilin-1 is a lab equipment product that functions to detect and quantify cofilin-1, a protein involved in the regulation of actin dynamics within cells.

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Lab products found in correlation

Alexa fluor secondary antibody, by Thermo Fisher Scientific (1 mentions) Fitc conjugated phalloidin, by Thermo Fisher Scientific (1 mentions) Anti actin, by Merck Group (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Bio-Rad (1 mentions) Rhodamine phalloidin, by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) Proteases inhibitor cocktail, by Merck Group (1 mentions) Hrp conjugated goat anti rabbit igg, by Southern Biotech (1 mentions) Chemiluminescence hrp substrate, by Merck Group (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Radioimmunoprecipitation assay (ripa), by Beyotime (1 mentions) Bca protein assay kit, by Keygen Biotech (1 mentions) Beyoecl kit, by Beyotime (1 mentions) Anti transgelin, by Abcam (1 mentions) Bsa assay kit, by Beyotime (1 mentions) Anti gelsolin, by Abcam (1 mentions) Anti hsp27, by Abcam (1 mentions) Anti gapdh, by Abcam (1 mentions)

4 protocols using anti cofilin 1

Immunoanalysis of Cytoskeletal Proteins

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Mouse monoclonal antibodies: anti-actin (Sigma Aldrich); anti-cofilin 1 (Abcam, Cambridge, MA). Rabbit polyclonal antibodies: anti-phosphorylated cofilin 1 (Santa Cruz Biotechnology, Santa Cruz, CA); anti-b-tubulin III (Covance, Emervylle, CA).
Fluorescent-conjugated Alexa Fluor secondary antibodies were from Molecular Probes (Eugene, OR); horseradish peroxidase-conjugated secondary antibodies (Bio-Rad, Hercules, CA); FITC-conjugated phalloidin (Molecular Probes-Life Technologies, Carlsbad, CA).

Tilve S., Difato F, & Chieregatti E. (2015). Cofilin 1 activation prevents the defects in axon elongation and guidance induced by extracellular alpha-synuclein. Scientific Reports, 5, 16524.

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Cofilin-1 and CAP1 Protein Analysis

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Standard immunocytofluorescence and western blot using anti-cofilin-1 or anti-CAP1 antibodies (Abcam) were performed. Detection of β-actin (Sigma antibody) was used as loading control in western blot. Rhodamine-phalloidin (Sigma) staining was used for actin visualization.

Bracalente C., Salguero N., Notcovich C., Müller C.B., da Motta L.L., Klamt F., Ibañez I.L, & Durán H. (2016). Reprogramming human A375 amelanotic melanoma cells by catalase overexpression: Reversion or promotion of malignancy by inducing melanogenesis or metastasis. Oncotarget, 7(27), 41142-41153.

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Western Blot Analysis of Cofilin 1

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Prepared cells or tissues were added with pre-cooling RIPA (Beyotime Bio, Shanghai, China) lysate buffer applied with proteases inhibitor cocktail (Sigma, USA). Protein concentration were measured using BCA Protein Assay kit (Keygen Biotech, Nanjing, China). Total proteins were mixed with 5× SDS-PAGE loading buffer and heat to 100 °C for 10 min, then, were separated with 4–15% SDS-PAGE and transferred to PVDF membranes (Millipore, USA). After blocked with 5% non-fat milk for 1 h, the PVDF membranes were incubated with specific primary and secondary (horseradish peroxidase, HRP-conjugated) antibodies. Finally, the protein bands were visualized using chemiluminescence HRP substrate (Millipore) in a dark room. The primary antibodies used are anti-GAPDH (1:10000, Kangchen, Shanghai, China) and anti-Cofilin 1 (1:1000, Abcam, USA). The secondary antibody used is HRP-conjugated goat anti-rabbit IgG (1:20000, Southern biotech, China).

Wang F., Wu D., Xu Z., Chen J., Zhang J., Li X., Chen S., He F., Xu J., Su L., Luo D., Zhang S, & Wang W. (2019). miR-182-5p affects human bladder cancer cell proliferation, migration and invasion through regulating Cofilin 1. Cancer Cell International, 19, 42.

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Proteomic Analysis of Aortic Tissue Lysates

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Aorta segment lysis was performed with chilled lysis buffer in presence of protease inhibitors. Lysate centrifugation was carried out at 12,000 rpm for 15 min at 4 °C. Total protein in the supernatant was quantitated with a BSA assay kit (P0006, Beyotime, Jiangsu, China). Protein separation was performed by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and protein bands were electro-transferred onto polyvinylidene difluoride (PVDF) membranes. The samples were incubated overnight at 4 °C with anti-tropomyosin β, anti-transgelin, anti-annexin, anti-gelsolin, anti-HSP-27, anti-cofilin-1 and anti-GAPDH (1:1000; Abcam, Cambridge, UK) primary antibodies. Further incubation was performed with goat anti-mouse or anti-rabbit secondary antibodies (1:10,000; Santa Cruz Biotechnology, USA) for 1h in ambient conditions. Development was carried out with the BeyoECL kit (Beyotime, China) and a Tanon 5200 system.

Wang H.Q., Meng X.Y., Zhang J.M., Chen J.Y., Zhang B.H, & Wu F.X. (2022). Alterations of actin cytoskeleton and arterial protein level in patients with obstructive jaundice. Genetics and Molecular Biology, 45(3), e20210419.

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