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Anti tgfβrii

Manufactured by Cell Signaling Technology
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Anti-TGFβRII is a primary antibody that recognizes the TGF-beta Receptor II protein. It is designed for use in various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to detect and analyze the expression and localization of TGF-beta Receptor II in biological samples.

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Lab products found in correlation

Gradient polyacrylamide gel, by Bio-Rad (2 mentions) Anti il 2rβ novus, by Novus Biologicals (2 mentions) Anti psmad3 ser423 ser425, by Cell Signaling Technology (2 mentions) Irdye 800cw anti rabbit igg, by LI COR (2 mentions) Anti mouse igg irdye 680lt, by LI COR (2 mentions) Image studio v2, by LI COR (2 mentions) Odyssey infrared imager, by LI COR (2 mentions) Anti smad3, by Cell Signaling Technology (2 mentions) Anti β actin, by Cell Signaling Technology (2 mentions) Anti caveolin, by Cell Signaling Technology (1 mentions) Oddyssey clx imaging system, by LI COR (1 mentions) Anti tgf β ri, by Abcam (1 mentions)

3 protocols using anti tgfβrii

1

Immunoprecipitation and Western Blot Analysis

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Ker-CT cultures were cultured and treated as described. Protein G Dynabeads Immunoprecipitation Kit was used according to manufactures protocol (ThermoFisher). Western blot was performed as described (25 (link)). Anti-caveolin (Cell Signaling, Danvers, MA), anti- TGF-βRII (Cell Signaling), anti-TGF-βRI (Abcam, Cambridge, MA), were used at the recommended dilution and IR labeled secondary antibody(s) (Licor, Lincoln, NE) were used at 1:10000 – 1:20,000. Blots were imaged via the Licor Oddyssey CLx imaging system (Licor).
Luckett-Chastain L.R., Cottrell M.L., Kawar B.M., Ihnat M.A, & Gallucci R.M. (2017). Interleukin (IL)-6 modulates transforming growth factor -β receptor I and II (TGF-βRI and II) function in epidermal keratinocytes. Experimental dermatology, 26(8), 697-704.
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2

Western Blot Analysis of Immune Signaling

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Cell lysates were electrophoresed on SDS-PAGE gels (4–15% gradient polyacrylamide gels, BioRad, Hercules, CA, USA) and blots of the gels were probed with the following antibodies: anti-IL-2Rβ Novus, Littleton, CO, USA), anti-TGFβRII, anti-pSTAT5 (Tyr694), anti-STAT5, anti-pSmad3 (Ser423/Ser425), anti-Smad3, and anti-β-actin (all Cell Signaling, Beverly, MA, USA). The secondary Ab used were anti-rabbit IgG IRDye 800CW and anti-mouse IgG IRDye 680LT (LI-COR, Lincoln, NE, USA). Membranes were imaged using the Odyssey Infrared Imager and quantified using Image Studio v2.0 software (LI-COR).
Sampson J.F., Suryawanshi A., Chen W.S., Rabinovich G.A, & Panjwani N. (2015). Galectin-8 promotes regulatory T cell differentiation by modulating IL-2 and TGFβ signaling. Immunology and cell biology, 94(2), 213-219.
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3

Western Blot Analysis of Immune Signaling

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Cell lysates were electrophoresed on SDS-PAGE gels (4–15% gradient polyacrylamide gels, BioRad, Hercules, CA, USA) and blots of the gels were probed with the following antibodies: anti-IL-2Rβ Novus, Littleton, CO, USA), anti-TGFβRII, anti-pSTAT5 (Tyr694), anti-STAT5, anti-pSmad3 (Ser423/Ser425), anti-Smad3, and anti-β-actin (all Cell Signaling, Beverly, MA, USA). The secondary Ab used were anti-rabbit IgG IRDye 800CW and anti-mouse IgG IRDye 680LT (LI-COR, Lincoln, NE, USA). Membranes were imaged using the Odyssey Infrared Imager and quantified using Image Studio v2.0 software (LI-COR).
Sampson J.F., Suryawanshi A., Chen W.S., Rabinovich G.A, & Panjwani N. (2015). Galectin-8 promotes regulatory T cell differentiation by modulating IL-2 and TGFβ signaling. Immunology and cell biology, 94(2), 213-219.
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