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Gelatin veronal buffer

Manufactured by Complement Technology
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Gelatin veronal buffer is a laboratory reagent used to prepare buffers for various biochemical and immunological applications. It is a mixture of sodium diethylbarbiturate (sodium barbital) and gelatin, which provides a stable and physiologically relevant pH range. This buffer is commonly used in processes such as electrophoresis, protein precipitation, and immunoassays.

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Lab products found in correlation

Eculizumab, by Thermo Fisher Scientific (2 mentions) Rat serum, by Complement Technology (2 mentions) Cynomolgus monkey, by BioIVT (2 mentions) Minipig, by BioIVT (2 mentions) Gelatin veronal buffer with calcium and magnesium gvb, by Complement Technology (2 mentions) Alexa fluor 488 nhs ester af488, by Thermo Fisher Scientific (2 mentions) Fluorescein isothiocyanate fitc labeled dextran, by Chondrex (2 mentions) Alexa fluor 647 dye, by Thermo Fisher Scientific (2 mentions) Fitc dextran, by Chondrex (2 mentions) Fixation and permeabilization buffer, by BioLegend (1 mentions) Facscanto, by BD (1 mentions)

Spelling variants of this product

Gelatin veronal buffer with calcium and magnesium (GVB++) (2 citations)

4 protocols using gelatin veronal buffer

1

Quantifying C4 Hemolytic Activity

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EA sheep red blood cells (Complement Technology) were washed with gelatin veronal buffer (Complement Technology) and counted with a spectrophotometer at 541 nm. To obtain 109 cells per ml, the following formula was used: final volume = initial volume × OD541/0.62. Cells were then diluted to 108 cells per ml, incubated with 2% C4-deficient guinea pig serum mixed with serial dilutions of fresh serum from hC4A/− and hC4B/− mice for 30 min at 37 °C. Absorbance was read at 415 nm. The percentage of hemolysis was measured based on 100% lysis control. In parallel, C4 concentration was measured by ELISA for each sample, and hemolytic activity per g of C4 was calculated and normalized to hC4A samples.
Yilmaz M., Yalcin E., Presumey J., Aw E., Ma M., Whelan C.W., Stevens B., McCarroll S.A, & Carroll M.C. (2020). Overexpression of schizophrenia susceptibility factor human complement C4A promotes excessive synaptic loss and behavioral changes in mice. Nature neuroscience, 24(2), 214-224.
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2

Synaptosomes Complement Activation Assay

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Synaptosomes were incubated with 10% fresh serum from C4−/−, hC4B/− or hC4A/− mice (diluted in half with C4−/− serum) for 30 min at 37 °C in gelatin veronal buffer (Complement Technology) then washed three times in PBS. Synaptosomes were fixed and permeabilized (fixation and permeabilization buffers from BioLegend) before antibody staining with anti-SV2 (DSHB/UIowa). For C4 detection, synaptosomes were stained with polyclonal rabbit anti-C4 (Dako) and acquired using a FACS Canto (BD Biosciences), and data were analyzed using FlowJo (Tree Star, v.10.4) software.
Yilmaz M., Yalcin E., Presumey J., Aw E., Ma M., Whelan C.W., Stevens B., McCarroll S.A, & Carroll M.C. (2020). Overexpression of schizophrenia susceptibility factor human complement C4A promotes excessive synaptic loss and behavioral changes in mice. Nature neuroscience, 24(2), 214-224.
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3

Complement Protein Binding Assay

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Antibody-sensitized sheep erythrocytes (EA), rabbit erythrocytes, normal human serum (NHS), C5-depleted or C9-depleted human serum, human complement proteins (including C5, C6, C7, C8, C9, and C5b6), and gelatin veronal buffer (GVB) and gelatin veronal buffer with calcium and magnesium (GVB++) were purchased from Complement Technology, Inc. (Tyler, TX, USA). Human C9 protein was conjugated with Alexa Fluor 647 dye using a labeling kit (Invitrogen, Carlsbad, CA, USA). Zilucoplan was prepared internally (UCB Ra Pharmaceuticals, Cambridge, MA, USA). eculizumab biosimilar was purchased from Syd Labs Inc. (Hopkinton, MA, USA). Labeling of eculizumab biosimilar with Alexa Fluor™ 488 NHS Ester (AF488) (Thermo Fisher, Waltham, MA, USA) was conducted using a labeling kit and following the vendor’s protocol (Supplementary Material). Fluorescein isothiocyanate (FITC)-labeled dextran (molecular weight approximately 4,000 and 150,000 Da; FITC-dextran 4 kDa and FITC-dextran 150 kDa, respectively) was from Chondrex (Woodinville, WA, USA).
Animal sera used in cross-species activity assay included rat serum (Complement Technology) and sera of baboon, beagle, chimpanzee, cynomolgus monkey, guinea pig, minipig, mouse, pig, rhesus monkey, and rabbit (all from BioIVT, Hicksville, NY, USA).
Tang G.Q., Tang Y., Dhamnaskar K., Hoarty M.D., Vyasamneni R., Vadysirisack D.D., Ma Z., Zhu N., Wang J.G., Bu C., Cong B., Palmer E., Duda P.W., Sayegh C, & Ricardo A. (2023). Zilucoplan, a macrocyclic peptide inhibitor of human complement component 5, uses a dual mode of action to prevent terminal complement pathway activation. Frontiers in Immunology, 14, 1213920.
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4

Complement Protein Binding Assay

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Antibody-sensitized sheep erythrocytes (EA), rabbit erythrocytes, normal human serum (NHS), C5-depleted or C9-depleted human serum, human complement proteins (including C5, C6, C7, C8, C9, and C5b6), and gelatin veronal buffer (GVB) and gelatin veronal buffer with calcium and magnesium (GVB++) were purchased from Complement Technology, Inc. (Tyler, TX, USA). Human C9 protein was conjugated with Alexa Fluor 647 dye using a labeling kit (Invitrogen, Carlsbad, CA, USA). Zilucoplan was prepared internally (UCB Ra Pharmaceuticals, Cambridge, MA, USA). eculizumab biosimilar was purchased from Syd Labs Inc. (Hopkinton, MA, USA). Labeling of eculizumab biosimilar with Alexa Fluor™ 488 NHS Ester (AF488) (Thermo Fisher, Waltham, MA, USA) was conducted using a labeling kit and following the vendor’s protocol (Supplementary Material). Fluorescein isothiocyanate (FITC)-labeled dextran (molecular weight approximately 4,000 and 150,000 Da; FITC-dextran 4 kDa and FITC-dextran 150 kDa, respectively) was from Chondrex (Woodinville, WA, USA).
Animal sera used in cross-species activity assay included rat serum (Complement Technology) and sera of baboon, beagle, chimpanzee, cynomolgus monkey, guinea pig, minipig, mouse, pig, rhesus monkey, and rabbit (all from BioIVT, Hicksville, NY, USA).
Tang G.Q., Tang Y., Dhamnaskar K., Hoarty M.D., Vyasamneni R., Vadysirisack D.D., Ma Z., Zhu N., Wang J.G., Bu C., Cong B., Palmer E., Duda P.W., Sayegh C, & Ricardo A. (2023). Zilucoplan, a macrocyclic peptide inhibitor of human complement component 5, uses a dual mode of action to prevent terminal complement pathway activation. Frontiers in Immunology, 14, 1213920.
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