Rauwolscine

Manufactured by Bio-Techne

Rauwolscine is a chemical compound derived from the Rauwolfia plant. It functions as an alpha-2 adrenergic receptor antagonist, which means it binds to and blocks the activity of a specific type of receptor in the body. This product is primarily used in scientific research and laboratory settings to study the role of alpha-2 adrenergic receptors in various biological processes.

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Rauwolscine hydrochloride (3 citations)

2 protocols using rauwolscine

Epitope-tagged Adrenergic Receptor Signaling

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The hemagglutinin (HA) epitope‐tagged α2a‐adrenergic receptor (HA‐α2a‐AR) used in this study was kindly provided by Dr. Joe Blumer (Medical University of South Carolina). Hemagglutinin epitope‐tagged RGS4 (HA‐RGS4) and FLAG‐tagged RGS14 (FLAG‐RGS14) were generated as described previously (Bernstein et al. 2004; Shu et al. 2007). The pertussis‐resistant mutants (C351G) of Gαo and Gαi1 were purchased from the cDNA Resource Center (cDNA.org, Bloomsberg, PA). Mas‐GRK3ct‐Luc and Ven‐Gβγ were described previously (Hollins et al. 2009). UK 14,304 was obtained from Sigma‐Aldrich (St. Louis, MO) while rauwolscine was purchased from Tocris Bioscience (Bristol, United Kingdom). Pertussis toxin was purchased from List Biological Laboratories, Inc (Campbell, CA).

Brown N.E., Lambert N.A, & Hepler J.R. (2016). RGS14 regulates the lifetime of Gα‐GTP signaling but does not prolong Gβγ signaling following receptor activation in live cells. Pharmacology Research & Perspectives, 4(5), e00249.

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Investigating PI3Kγ Signaling Pathways

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Human SDF1α was purchased from PeproTech; UK14304, GW5074, rapamycin, BFA, ilimaquinone, monensin, nigericin, swainsonine, and LY294002 were from Sigma Aldrich; nocodazole, insulin-like growth factor 1, AMD3100, control siRNA (medium GC), siRNAs targeting to human PI3Kγ regulatory subunits p101 and p87, and antibodies against GFP, phospho-ERK1/2, Gγ9, and β-actin and the PI3Kγ subunits p110γ, p101 and p87 were from Santa Cruz Biotechnology; wortmannin, AS-604850, and GSK2292767 were from ApexBio; TGX-221 and HS-173 were from Adooq Bioscience; U0126 and PD98059 were from Calbiochem; EGF, puromycin, and blasticidin S were from Thermo Fisher Scientific; U-73122 and AZD5363 were from MedChemExpress; U-73433, CRT0066101, and Go6976 were from Cayman Chemical; PTX was from List Biological Laboratories; gallein and rauwolscine were from Tocris Bioscience; D-Luciferin was from GoldBio; antibodies against hemagglutinin and ERK1/2 were from Cell Signaling Technology; antibodies against Gγ3 were from Abcam. All other materials were obtained as described (52 (link), 53 (link)).

Khater M., Wei Z., Xu X., Huang W., Lokeshwar B.L., Lambert N.A, & Wu G. (2021). G protein βγ translocation to the Golgi apparatus activates MAPK via p110γ-p101 heterodimers. The Journal of Biological Chemistry, 296, 100325.

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