Five sections spanning the entire brain were stained with a 6E10 antibody (1:1000, Biolegend) or Congo red (Sigma) to detect Aβ plaques in the brain (the 6E10 antibody was used to stain compact and diffuse Aβ; Congo red was used to stain for compact Aβ). CAA was manually assessed in Congo red‐stained hippocampal sections under a microscope (Wilcock et al., 2006 (link)).
6e10 antibody
Manufactured by Merck Group
The 6E10 antibody is a lab equipment product developed by Merck Group. It is a well-characterized monoclonal antibody that specifically recognizes the amyloid-beta protein. The 6E10 antibody can be utilized in various research applications that require the detection or quantification of amyloid-beta.
Automatically generated - may contain errors
Lab products found in correlation
Congo red, by Merck Group (2 mentions)
6e10 antibody, by BioLegend (1 mentions)
Anti bdnf antibody, by Abcam (1 mentions)
Brain derived neurotrophic factor (bdnf), by Thermo Fisher Scientific (1 mentions)
Vitamin e, by Merck Group (1 mentions)
Hexafluoroisopropanol, by Merck Group (1 mentions)
Donepezil, by Merck Group (1 mentions)
Anti mn sod antibody, by Merck Group (1 mentions)
Anti cd68 antibody, by Abcam (1 mentions)
Caspase 3, by Abcam (1 mentions)
3 protocols using 6e10 antibody
1
Immunohistochemical Detection of Amyloid-Beta
2
Alzheimer's Disease Therapeutic Evaluation
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PSM-04 was provided by Braintropia (Korea) for research purposes. BDNF was purchased from Peprotech (United States). Donepezil, 6E10 antibody, hexafluoroisopropanol (HFP), and vitamin E were purchased from Sigma Aldrich (St. Louis, MO, United States). Anti-BDNF antibody was purchased from Abcam (Cambridge, United Kingdom), and anti-Mn-SOD antibody was purchased from Merck Millipore (Darmstadt, Germany).
3
Characterizing Alzheimer's-Related Pathology
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Immunohistochemistry and immuno uorescence were conducted to our previous methods (13) . Serial coronal brain sections (30 µm in thickness) were cut using cryostat. Five brain sections spanning the entire brain were stained with a 6E10 antibody or Congo red (Sigma) to detect Aβ plaques in the brain (6E10 antibody for compact and diffuse Aβ; Congo red for compact Aβ). Cerebral amyloid angiopathy (CAA) was manually assessed in Congo red-stained hippocampal sections under a microscope (14) . Brain sections were also incubated with anti-CD68 antibody (Abcam) and anti-glial brillary acidic protein (GFAP) antibody (Abcam) to assess the microgliosis and astrocytosis, respectively. Double immuno uorescence staining for NeuN and microtubule associated protein (MAP)-2 (Abcam) were performed to detect the neuronal loss and neurite degeneration. Apoptosis of neurons in the CA3 area of hippocampus was assessed by double-immuno uorescence staining for NeuN (Abcam) and caspase-3 (Abcam).
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