Il 1β

Manufactured by Dakewe

Sourced in China, United States

IL-1β is a cytokine that plays a key role in the inflammatory response. It is involved in the regulation of immune responses, cell proliferation, and differentiation.

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Lab products found in correlation

Tnf α, by Dakewe (9 mentions) Interleukin 6 (il 6), by Dakewe (5 mentions) Il 10, by Dakewe (3 mentions) Ifn γ, by Dakewe (2 mentions) Mouse il 1β, by R&D Systems (2 mentions) Il 12, by Dakewe (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Artemisinin, by Merck Group (1 mentions) Iba 1 ab5076, by Abcam (1 mentions) Bv2 microglial cells, by Jennio Biotech (1 mentions) Mcp 1, by Dakewe (1 mentions) Lipopolysaccharide lps, by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Alexa fluor 546 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 donkey anti mouse igg h l, by Thermo Fisher Scientific (1 mentions) Anti phospho iκbα, by Cell Signaling Technology (1 mentions) Anti cox 2, by Santa Cruz Biotechnology (1 mentions) Anti nlrp3, by Abcam (1 mentions)

Spelling variants of this product

Mouse IL-1β ELISA kit (5 citations) Human IL-1β ELISA Kit (4 citations) IL-1β ELISA kit (3 citations) Mouse IL-1β (3 citations) Human IL-1β (2 citations) Rat IL-1β ELISA Kit (2 citations)

15 protocols using il 1β

Artemisinin Modulates Microglial Activation

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BV2 microglial cells were purchased from Guangzhou Jennio Biotech Co., Ltd (Guangzhou, China). Artemisinin (purity > 98%), LPS and DMSO were from Sigma Aldrich (MO, USA). Iba-1 (ab5076) and TNF-α (ab1793) antibodies were purchased from Abcam (Cambridge, UK). AMPKα1 (cst 2795s), p-AMPKα1 (cst 4185s), iNOS (cst 13120s), IL-6 (cst 12912), NF-κB (cst 8242s), PCNA (cst 13100s), α-Tubulin (cst 2125s) and β-actin (cst 4970s) antibodies were purchased from Cell Signaling Technology Inc (Danvers, USA).
Cleaved caspase 3 (GB11532) and CD68 (GB11067) antibodies was purchased from Wuhan Servicebio Biotechnology, Co., Ltd. (Wuhan, China). P2Y12 antibody (PAB32958) was purchased from Biowamp Life Science Lab, Co., Ltd. (Wuhan, China). TNF-α, IL-6, IL-1α, IL-1β, MCP-1 and MIP-2 ELISA kits were purchased from Dakewe Bio-engineering Co., Ltd (Shenzhen, China).

Lin S., Wei J., Ye S., Hu J., Bu J., Zhu L., Li Q., Liao H., Wu Y., Lin P., Chen S., & Chen X. (2020). Artemisinin improves neurocognitive deficits associated with sepsis by activating the AMPK axis in the microglia.

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Evaluation of MI-2 and Mepazine Compounds

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MI-2 and mepazine (chemical structure shown in Figure 1A, synthetic compounds provided by Eternity Bioscience Inc. NJ, USA) was dissolved at a concentration of 30 mM in 100% DMSO as a stock solution, stored at −20°C, and diluted with medium before each experiment. The final DMSO concentration did not exceed 0.1% throughout the study (all the control groups are composed of 0.1% DMSO). Cyclosporine A (CsA), phorbol myristate acetate (PMA), lipopolysaccharide (LPS) and adenosine triphosphate (ATP) were purchased from Sigma-Aldrich (St. Louis, MO). Dextran sulfate sodium (DSS, 36–50 kDa) was bought from MP Biomedicals (Aurora, OH). Myeloperoxidase (MPO) activity assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). RPMI-1640, FBS, Alexa Fluor 546 Donkey Anti-Rabbit IgG and Alexa Fluor^® 488 Donkey Anti-Mouse IgG (H+L) were purchased from Life technology (Carlsbad, CA). Anti-phospho-IκBα, anti-phospho-IKKα/β, were purchased from Cell Signaling Technology (Beverly, MA). Anti-NLRP3, anti-phospho-p65 and anti-CASP1 were purchased from Epitomics (Burlingame, CA). Anti-ASC and anti-COX2 were purchased from Santa Cruz (Santa Cruz, CA). ELISA kits for murine TNF, IL-1β, IL-6, IFN-γ and human IL-1β were purchased from Dakewe Biotech Co. Ltd (Beijing, China). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO).

Liu W., Guo W., Hang N., Yang Y., Wu X., Shen Y., Cao J., Sun Y, & Xu Q. (2016). MALT1 inhibitors prevent the development of DSS-induced experimental colitis in mice via inhibiting NF-κB and NLRP3 inflammasome activation. Oncotarget, 7(21), 30536-30549.

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Cytokine Quantification in Cell Culture and Serum

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Mouse IL-1β (R&D Systems, Minneapolis, MN, United States), IL-1β, TNF-α, and IL-6 (Dakewe, Beijing, China), according to the manufacturer’s instructions, were used to measure the cell culture supernatants and mouse serum, respectively.

Gao Y., Shi W., Tu C., Li P., Zhao G., Xiao X., Wang J, & Bai Z. (2022). Immunostimulatory activity and structure-activity relationship of epimedin B from Epimedium brevicornu Maxim.. Frontiers in Pharmacology, 13, 1015846.

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Evaluating Inflammatory Responses via Caspase-1, Cytokines, and Liver Enzymes

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The Caspase-Glo® 1 In ammasome Assay (Promega, Madison, WI, USA) was used to assess caspase-1 activity in cell culture supernatants according to the manufacturer's instructions.
Enzyme-linked immunosorbent assay (ELISA)
Cell culture supernatants and mouse serum were analyzed for mouse IL-1β (R&D Systems, Minneapolis, MN, USA), IL-1β, TNF-α, and IL-6 (Dakewe, Beijing, China), respectively, according to the manufacturer's instructions.
Alanine aminotransferase (ALT) and aspartate transaminase (AST)
Serum ALT and AST levels were determined according to the Nanjing Jiancheng Bioengineering Institute (Nanjing, China) and the Nanjing Jiancheng Bioengineering Institute (GOT) assay kit instructions [23] .

Gao Y., Shi W., Tu C., Zhao G., Xu G., Xiao X., & Bai Z. (2021). Epimedin B Through Enhancing NLRP3 Inflammasome Activation To Induces Idiosyncratic Hepatotoxicity.

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Cytokine Level Assessment in Serum

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Blood samples were collected to acquire the serum. The concentrations of IL‐1β, IL‐6 and TNF‐α were measured with enzyme‐linked immunosorbent assay (ELISA) in accordance with the manufacturer’s protocols for IL‐1β, IL‐6 and TNF‐α kits respectively (Dakewe Biotech Co., Ltd., Shenzhen, China). The sensitivity of the assay was 22 pg ml⁻¹ for IL‐1β, 7 pg ml⁻¹ for IL‐6 and 8 pg ml⁻¹ for TNF‐α. Intra‐ and interassay coefficients of variation were < 10% and < 15% respectively, for IL‐1β, IL‐6 and TNF‐α.

Zhu H., Li W., Wang Z., Chen J., Ding M, & Han L. (2019). TREM‐1 deficiency attenuates the inflammatory responses in LPS‐induced murine endometritis. Microbial Biotechnology, 12(6), 1337-1345.

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LPS-Induced Inflammatory Response in BV-2 Cells

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BV-2 cells were exposed to 1 μg/ml LPS for 6 h to stimulate the inflammatory response. For the LPS groups, cells incubated with culture medium served as the control. BV-2 microglial cells in the LPS + PK11195 or LPS + midazolam groups were pretreated with 0.5 μM PK11195 or 15 μM midazolam for 1 h and then exposed to LPS for 6 h. Subsequently, the medium of the different groups was collected. IL-1β (Dakewe, Beijing, China) and IL-18 (Multi Sciences, Hangzhou, Zhejiang, China) that the cells had secreted into the culture supernatant were measured by ELISAs according to the manufacturer’s instructions. The optical density (OD) values at 450 nm were measured using a microplate reader.

Feng H., Liu Y., Zhang R., Liang Y., Sun L., Lan N, & Ma B. (2020). TSPO Ligands PK11195 and Midazolam Reduce NLRP3 Inflammasome Activation and Proinflammatory Cytokine Release in BV-2 Cells. Frontiers in Cellular Neuroscience, 14, 544431.

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Engineered IFN-γ and OmpA Peptide Protocol

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The IFN-γ gene and OmpA peptide gene sequence was obtained from GenBank and synthesized by Sangon bioengineering Co.(Shanghai, China). The pBV220 plasmid was purchased from Miaoling Bioscience & Technology Co.(Wuhan, China). E. coli DH5α strain and E. coli MG1655 strain were purchased from Angyubio Biotechnology Co. (Shanghai, China). The mouse breast cancer cells (4T1) were obtained from American Type Culture Collection. Dulbecco’s modified Eagle’s medium (DMEM) culture medium, fetal bovine serum (FBS), penicillin and streptomycin solution, trypsin containing 0.25% EDTA were purchased from Gibco (USA). 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide (DiR), live & dead cell viability detection kit and CCK-8 assay kit were obtained from Beyotime Biotechnology Co. (Shanghai, China). Murine IFN-γ, TNF-α, IL-10, IL-1β and TGF-β enzyme-linked immunosorbent assay (ELISA) kits were obtained from Dakewe Bioengineering Co. (Shenzhen, China).

Chen Y., Du M., Yuan Z., Chen Z, & Yan F. (2022). Spatiotemporal control of engineered bacteria to express interferon-γ by focused ultrasound for tumor immunotherapy. Nature Communications, 13, 4468.

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Retinal Inflammation and Angiogenic Markers

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The supernatant of the retinal homogenate was collected after centrifugation at 4270 × g for 20 minutes. IL-1β (Dakewe Biotech Co., Ltd., Shenzhen, China), IL-18 (Dakewe Biotech Co., Ltd.), tumor necrosis factor-α (TNF-α; Jiancheng Biotech Co., Ltd., Nanjing, China) and IL-6 (Jiancheng Biotech Co., Ltd.) were chosen as inflammatory markers in the retinas. VEGF (Boster Biological Technology Co. Ltd., Wuhan, China) and sICAM-1 (Ruishuo Biotechnology Co., Ltd., Shanghai, China) were selected as pro-angiogenic factors. BDNF (R&D Systems, Minneapolis, MN, USA) and NGF (R&D Systems) are neurotrophic cytokines. They were detected according to the corresponding ELISA kit instructions.

Chai G.R., Liu S., Yang H.W, & Chen X.L. (2020). Quercetin protects against diabetic retinopathy in rats by inducing heme oxygenase-1 expression. Neural Regeneration Research, 16(7), 1344-1350.

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Cytokine Profiling of hCMSCs with LPS and Liraglutide

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hCMSCs were seeded with 0, 1, 10, 30, and 50 mg/ml LPS alone or with 10 nM liraglutide after 48 h. The culture media were then collected and detected for the secretion of many cytokines such as TNF-α (Dakewe, Cat. No. DKW12-2720-096, China), IL-1β (Dakewe, Cat. No. DKW12-2012-096, China), IL-6 (Dakewe, Cat. No. DKW12-2060-096, China), IL-10 (Neobioscience, Cat. No. EMC005, China), and a rat cAMP ELISA kit (Jiang Lai Biotechnology, JL10117, China) following the instruction of the ELISA kit.

Feng Y., Wang L., Ma X., Yang X., Don O., Chen X., Qu J, & Song Y. (2020). Effect of hCMSCs and liraglutide combination in ALI through cAMP/PKAc/β-catenin signaling pathway. Stem Cell Research & Therapy, 11, 2.

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Quantifying Galectin-9 and Inflammatory Markers

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The culture supernatant of second to fifth generation MSCs, and MSCs with or without IFN-γ stimulation were collected to test Gal-9 expression. Furthermore, the liver, lung, and kidney tissue homogenate were also obtained to detect Gal-9 expression level (BaoLai biological technology, Jiangsu, China). In addition, the serum and liver homogenate were gathered, respectively, to detect the expression level of TNF-α and IL-1β (DAKEWE, Shenzhen, China). According to the manufacturer’s instructions, the detection antibody, HRP conjugate, chromogenic substrate, and stop solution were added in order. Finally, the absorbance of each well was detected at 450 nm, and the concentration of each sample was obtained by comparing with the standard curve. All tests were performed in duplicate to eliminate the error.

Zhao Y., Yu D., Wang H., Jin W., Li X., Hu Y., Qin Y., Kong D., Li G., Ellen A, & Wang H. (2022). Galectin-9 Mediates the Therapeutic Effect of Mesenchymal Stem Cells on Experimental Endotoxemia. Frontiers in Cell and Developmental Biology, 10, 700702.

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