Twist sc 15393

Human colorectal adenocarcinoma cell lines HCT116 and SW480 were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA). Antibodies against PARP-1 (sc-8007), PCNA (sc-56), α-tubulin (sc-5286), ZO-1 (sc-33725), p-GSK3β (sc-135653), and Twist (sc-15393) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies against claudin (#132552), p-Akt (#9271), Slug (#9585), Snail (#3879), N-cadherin (#13116), E-cadherin (#3196), and p-Smad (#9511) were purchased from Cell Signaling Technology (Beverly, MA, USA). Recombinant human TGF beta 1 protein (TGF-β) was purchased from Abcam (Cambridge, UK).

Monoclonal antibodies to N-cadherin (610920) and E-cadherin (612130) were purchased from BD Transduction Laboratory (Seoul, Korea). Polyclonal antibodies to STAT3 (sc-482), NF-κB (p65) (sc- 109), Vimentin (sc-5565), SLUG (sc-10436) and TWIST (sc- 15393) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The polyclonal antibody to ZEB1 (HPA027524) was purchased from Sigma (St Louis, MO, USA). The polyclonal antibodies to Snail (3879), p-STAT3 (Y705) (9131), p-STAT3 (S727) (9134), Fra1 (5281), p-Fra1 (3880) and Notch1 (2421) were obtained from Cell Signaling Technology (Beverly, MA, USA). Polyclonal antibodies to anti-IL-6 (ab6672), Notch2 (ab72803), Jagged1 (ab109536) and DLL4 (ab7280) were purchased from Abcam (Cambridge, UK. Monoclonal antibody to β-actin and 4, 6-diamidino-2-phenylindole (DAPI) were purchased from Sigma (St Louis, MO, USA). The chemical inhibitors including Gamma secretase inhibitor (565770), STAT3 inhibitor (573097) and the JAK inhibitor (420099) were bought from Calbiochem (San Diego, CA, USA). Recombinant human CXCL8/IL-8 protein was purchased from R & D systems and recombinant protein IL-6 was bought from EMD Millipore.