Anti human igg hrp

Manufactured by Promega

Anti-human IgG-HRP is a labeling reagent that binds to human immunoglobulin G (IgG) antibodies and is conjugated to the enzyme horseradish peroxidase (HRP). It is designed for use in various immunoassay applications.

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Lab products found in correlation

3 3 5 5 tetramethylbenzidine (tmb), by Thermo Fisher Scientific (1 mentions) Goat anti human igg a488, by Jackson ImmunoResearch (1 mentions) Anti human igg dylight488, by Abcam (1 mentions) Bacteroides thetaiotaomicron, by Leibniz Institute DSMZ (1 mentions) Microplate reader, by Bio-Rad (1 mentions) Bis tris sds page gel, by Thermo Fisher Scientific (1 mentions) Sambucus nigra lectin sna, by Vector Laboratories (1 mentions) Protein free blocking buffer, by Thermo Fisher Scientific (1 mentions) Anti rabbit igg hrp, by Promega (1 mentions)

Spelling variants of this product

Anti-Human IgG-HRP Conjugate (3 citations) HRP-conjugated goat anti-human IgG (2 citations) Anti-human IgG (H+L) HRP conjugate antibody (2 citations) HRP-conjugated anti-human IgG (2 citations) HRP conjugated anti-human IgG secondary antibody (2 citations)

4 protocols using anti human igg hrp

Pharmacokinetics of Fc-Fused Proteins

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50μg of human IgG-Fc, B4^Fc, ST6^Fc or B4ST6^Fc was intravenously administered to C57BL/6 female mice. The mice they were bled 1, 2, 4, 6, 8, 10 days after the injection. Injected protein in mice sera was detected by ELISA. Briefly, 96-well plates were coated with 5μg/ml of anti-human IgG Fc (Bethyl Laboratories), blocked with 2% BSA in PBS, and probed with anti-human IgG-HRP (20ng/ml, Promega). 3,3,5,5-tetramethylbenzidine (TMB; Thermo Fisher Scientific) was used for the detection, and 2M sulfuric acid was used to stop the reaction.

Pagan J.D., Kitaoka M, & Anthony R.M. (2017). Engineered Sialylation of Pathogenic Antibodies In Vivo Attenuates Autoimmune Disease. Cell, 172(3), 564-577.e13.

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Immunoassay Reagents and Bacterial Cultures

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Goat anti-human IgG A488 and goat anti-human IgM A647 were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA). Anti-human IgG DyLight488 and anti-human IgM DyLight488 were obtained from Abcam (Cambridge, MA). Anti-human IgG HRP was from Promega (Fitchburg, WI). Bacteroides vulgatus (DSM 1447), Bacteroides intestinalis (DSM 17393), and Bacteroides thetaiotaomicron (DSM 2079) were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany).

Kappler K., Restin T., Lasanajak Y., Smith D.F., Bassler D, & Hennet T. (2020). Limited Neonatal Carbohydrate-Specific Antibody Repertoire Consecutive to Partial Prenatal Transfer of Maternal Antibodies. Frontiers in Immunology, 11, 573629.

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HIV-1 Envelope Glycoprotein Binding Assay

Cited 1 time

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Trimeric ectodomain of NL4-3 (subtype B) constructed based on BG505 SOSIP.664 [42 (link)] was kindly provided by Dr. Yi Shi at Institute of Microbiology, Chinese Academy of Sciences. Monomeric gp120 and monomeric gp140 (CRF01_AE) derived from a CRF01_AE circulating strain CM235 (Genebank #: AAG28611) isolated in Thailand in year 2000 were purchased and produced from 293T cells (Immune Technology Corp, China). Recombinant envelope glycoprotein was coated overnight at 4 °C on the 96-well plate (100 ng/well), blocked for 2 h at 37 °C with 1 % bovine serum albumin (BSA) in PBS before addition of ten serial threefold dilutions of plasma samples. After incubating for 1 h at 37 °C and three-time thorough washes with PBST (PBS with 0.05 % Tween), the secondary antibody conjugated with horseradish peroxidase (1:4000 dilution, anti-human IgG-HRP, Promega) was added before applying substrate for detectioin. Maximum absorbance at 450 nm and corresponding plasma dilution were recorded (Microplate Reader, Bio-Rad). Plasma samples from HIV-1 negative individuals were included as negative controls.

Jin S., Ji Y., Wang Q., Wang H., Shi X., Han X., Zhou T., Shang H, & Zhang L. (2016). Spatiotemporal hierarchy in antibody recognition against transmitted HIV-1 envelope glycoprotein during natural infection. Retrovirology, 13, 12.

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Western and Lectin Blot Protocols

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Western and lectin blots were performed as described previously (Anthony et al., 2008a (link)). Briefly, equal amounts of protein were resolved on 4 – 12% Bis-Tris SDS-PAGE gel (Life Technologies) and then transferred to polyvinylidene difluoride membranes. After blocking the membranes with 5% dry milk in PBST (0.05% Tween 20) for western blot, proteins were detected using either anti-human IgG-HRP (20ng/ml, Promega); anti-human B4GALT1 (100ng/ml, Sigma-Aldrich) followed by anti-rabbit IgG-HRP (50ng/ml, Promega); or anti-human ST6GAL1 sera (1:100, generous gift from Dr. J. Paulson) followed by anti-rabbit IgG-HRP. For lectin blots, the membranes were blocked in Protein Free Blocking Buffer (Thermo Fisher Scientific), and probed with either biotinylated Sambucus Nigra Lectin (SNA; 5μg/ml, Vector Laboratories) or with biotinylated Erythrina Cristagalli Lectin (ECL; 5μg/ml, Vector Laboratories) to detect terminal sialic acid or galactose, respectively.

Pagan J.D., Kitaoka M, & Anthony R.M. (2017). Engineered Sialylation of Pathogenic Antibodies In Vivo Attenuates Autoimmune Disease. Cell, 172(3), 564-577.e13.

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