Fitc conjugated p24 gag 6604665 antibody

Cocaine hydrochloride was procured from Sigma-Aldrich Co. (St. Louis, MO), DC-SIGN, LAMP1, VPS4 and SAMHD1 antibodies were obtained from Cell Signaling Technology (Danvers, MA). APOBEC3G, CD81, Tetherin and GAPDH antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). LARG and 4G10 antibodies were obtained from EMD Millipore (Billerica, MA), Rhodamine-Phalloidin (R415) and CellTrace Far-Red DDAO-SE (C34553) were obtained from Life Technologies Corp. FITC-conjugated p24 GAG (6604665) antibody was obtained from Beckman Coulter, Inc. (Brea, CA). PerCP/Cy5.5-conjugated CXCR4, FITC-conjugated CCR5 and AlexaFluor 647-conjugated DC-SIGN antibodies were obtained from BioLegend (San Diego, CA). Anti-GFP antibody (FITC) was obtained from Abcam (Cambridge, MA).

All reagents used in this study were molecular grade and procured from Sigma-Aldrich Co. (St. Louis, MO), unless otherwise indicated. Recombinant Slit2N was from Peprotech (Rocky Hill, NJ). The Slit2 (ab7665) and Robo1 (ab85312) antibodies were obtained from Abcam, Inc. Phosphorylated c-Src (2466) and Cdc42 (6943) antibodies were obtained from Cell Signaling Technology (Danvers, MA). Actin (SC8432), Arp2 (SC137250), WASp (SC365859), Fli1 (SC30046, SC21716), Dia2 (SC55539), GAPDH (SC25778) and FITC-conjugated actin (SC1615FITC) antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Rhodamine-Phalloidin (R415) and CellTrace Far-Red DDAO-SE (C34553) were obtained from Life Technologies Corp. FITC-conjugated p24 GAG (6604665) antibody was obtained from Beckman Coulter, Inc. (Brea, CA). DC-SIGN and CD1a antibodies were obtained from R&D Systems, Inc. (Minneapolis, MN).

HIV-1 BaL was obtained from the NIH AIDS Research and Reference Reagent Program, National Institute of Allergy and Infectious Diseases, NIH. The HIV-1 BaL strain has been shown to efficiently infect, via the CCR5 co-receptor, and replicate within CD4⁺ T-cells^{49 (link)}. Argonaute-1, p-NFκB, NFκB, p-ERK, p-Src, p-Akt, NFAT1, p-CREB, CREB, Oct-1, Ubiquitin, and β-Actin antibodies were obtained from Cell Signaling Technology (Danvers, MA). GW182, D1DR, D2DR, D3DR, D4DR, Sigma-1 Receptor, and GAPDH antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). FITC-conjugated p24 GAG (6604665) antibody was obtained from Beckman Coulter, Inc. (Brea, CA). PE-conjugated CD45RO, PE-conjugated HLA-DR, FITC-conjugated CD45RA, FITC-conjugated CD25, FITC-conjugated CD69, PE isotype control, and FITC isotype control antibodies were purchased from Biolegend (San Diego, CA). p-NFAT1 antibody was obtained from Thermo Fisher Scientific (Waltham, MA). Fluo-4, AM was purchased from Invitrogen (Carlsbad, CA). Methamphetamine hydrochloride and sigma-1 receptor inhibitor (BD1047) were purchased from Sigma Aldrich (St. Louis, MO).