Optical rotations, IR, and UV spectra were recorded on a JASCO P-1020 polarimeter (Tokyo, Japan), Hercules Bio-Rad FTS-135 series spectrometer (CA, USA), and Shimadzu UV-2401 PC ultraviolet–visible spectrophotometer (Tokyo, Japan), respectively. Electrospray ionization mass spectra (ESI-MS) and high-resolution electrospray ionization mass spectra (HR-ESI-MS) were carried out on an Agilent G6230 TOF MS spectrometer. 1D- and 2D-NMR spectra were recorded on a Bruker DRX-500, 600 or 800 spectrometer (Karlsruhe, Germany). The X-ray diffraction instrument (Bruker APEX DUO, Karlsruhe, Germany) was used for characterizing the structures of the crystal. Macroporous resin D101 and silica gel (200–300 mesh) were used for column chromatography (CC), and TLC analysis with silica gel GF254 plates were visualized by heating after spraying with 10% H2SO4/EtOH. All CC and TLC materials were purchased from Qingdao Haiyang Chemical Co. Ltd., China. Reverse-phase semipreparative HPLC (Capcell Pak MGII C18 column, 5 μm, 250 mm × 10 mm, Tokyo, Japan) was performed on a Hanbon series (Jiangsu Hanbon Science & Technology Co., China) at 25 °C with a flowing rate of 3.0 mL/min.
G6230 tof ms spectrometer
Manufactured by Agilent Technologies
Sourced in United States
The G6230 TOF MS spectrometer is a mass spectrometer produced by Agilent Technologies. It utilizes time-of-flight (TOF) technology to analyze the mass-to-charge ratio of ionized molecules. The instrument measures the time it takes for these ions to travel through a vacuum tube and reach the detector, providing information about their molecular weights.
Automatically generated - may contain errors
Lab products found in correlation
1260 liquid chromatography system, by Agilent Technologies (2 mentions)
Sephadex lh 20, by Cytiva (2 mentions)
Sb c18 column, by Agilent Technologies (2 mentions)
Avance 3 600 mhz spectrometer, by Bruker (2 mentions)
Sepacore system, by Büchi (2 mentions)
C 615, by Büchi (2 mentions)
Silica gel 200 300 mesh, by Qingdao Marine Chemical (2 mentions)
C 605, by Büchi (2 mentions)
Apex duo, by Bruker (1 mentions)
P 1020 digital polarimeter, by Jasco (1 mentions)
Uv 2401pc instrument, by Shimadzu (1 mentions)
Autospec premier p776 spectrometer, by Waters Corporation (1 mentions)
Xevo tqs spectrometer, by Waters Corporation (1 mentions)
Avance 3 700 mhz spectrometer, by Bruker (1 mentions)
Uv 2401pc spectrometer, by Shimadzu (1 mentions)
Sepa 300 polarimeter, by Horiba (1 mentions)
Zorbax sb c18, by Agilent Technologies (1 mentions)
Mci gel chp 20p, by Mitsubishi (1 mentions)
Autospec premier p776 mass spectrometer, by Waters Corporation (1 mentions)
Tensor 27 infrared spectrophotometer, by Bruker (1 mentions)
4 protocols using g6230 tof ms spectrometer
1
Spectroscopic and Structural Characterization
2
Spectroscopic Characterization of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were obtained on a JASCO P-1020 digital polarimeter. IR and UV spectra were recorded on a Bruker Tensor 27 FT-IR spectrometer with KBr pellets and a Shimadzu UV-2401PC instrument, respectively. 1D and 2D NMR spectra were obtained on a Bruker Avance III 600 MHz spectrometer. ESIMS and HREIMS were measured on a Waters Xevo TQ-S spectrometer and a Waters Autospec Premier P776 spectrometer, respectively. HRESIMS was measured on an Agilent G6230 TOF MS spectrometer. Silica gel 200–300 mesh (Qingdao Marine Chemical Inc., China) and Sephadex LH-20 (Amersham Biosciences, Sweden) were used for column chromatography. Medium pressure liquid chromatography (MPLC) was performed on a Büchi Sepacore System equipping pump manager C-615, pump modules C-605 and fraction collector C-660 (Büchi Labortechnik AG, Switzerland), and columns packed with Chromatorex C-18 (40–75 μm, Fuji Silysia Chemical Ltd., Japan). Preparative high performance liquid chromatography (Prep-HPLC) was performed on an Agilent 1260 liquid chromatography system equipped with a Zorbax SB-C18 column (5 μm, 9.4 × 150 mm).
3
Analytical Techniques for Chemical Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were measured with a Horiba SEPA-300 polarimeter (Horiba, Kyoto, Japan). UV spectra were recorded on a Shimadzu UV-2401PC spectrometer (Shimadzu Corporation, Tokyo, Japan). For column chromatography, C-18 silica gel (40 μm × 63 μm; Daiso Co., Japan), MCI gel CHP 20P (75 μm × 150 μm, Mitsubishi Chemical Industries, Tokyo, Japan), and Sephadex LH-20 (Amersham Pharmacia, Uppsala, Sweden) were employed. Semipreparative HPLC was performed using an Agilent Zorbax SB-C18 (250 mm × 9.4 mm, i.d., 5 μm). NMR spectra were recorded on a Bruker Avance III 700 MHz spectrometer (Bruker, Karlsruhe, Germany), or a Bruker Avance III 600 MHz spectrometer, and TMS was used as an internal standard. HREIMS data were collected with an Agilent G6230 TOF MS spectrometer (Agilent Technologies, Santa Clara, CA, United States).
4
Spectroscopic Analysis of Natural Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
UV spectra were obtained using a Shamashim UV 2401 spectrometer. Optical
rotations were recorded on a JASCO P-1020 polarimeter. IR spectra were measured on
a Bruker Tensor-27 infrared spectrophotometer with KBr pellets. HREIMS were
obtained on a Waters Autospec Premier P776 mass spectrometer. HRESIMS were taken
on an Agilent G6230 TOF MS spectrometer. 1D and 2D NMR spectra were recorded on
Bruker Avance-600 and Ultrashield-800 spectrometers using TMS as an internal
standard. Silica gel 200–300 mesh (Qingdao Marine Chemical Inc., China) and
Sephadex LH-20 (Amersham Biosciences, Sweden) were used for column chromatography.
Medium pressure liquid chromatography (MPLC) was performed on a Büchi Sepacore
System equipping pump manager C-615, pump modules C-605 and fraction collector
C-660 (Büchi Labortechnik AG, Switzerland), and columns packed with Chromatorex
C-18 (40–75 μm, Fuji Silysia Chemical Ltd., Japan). Preparative HPLC was performed
on an Agilent 1260 liquid chromatography system equipped with a Zorbax SB-C18
column (5 μm, 9.4 mm × 150 mm).
rotations were recorded on a JASCO P-1020 polarimeter. IR spectra were measured on
a Bruker Tensor-27 infrared spectrophotometer with KBr pellets. HREIMS were
obtained on a Waters Autospec Premier P776 mass spectrometer. HRESIMS were taken
on an Agilent G6230 TOF MS spectrometer. 1D and 2D NMR spectra were recorded on
Bruker Avance-600 and Ultrashield-800 spectrometers using TMS as an internal
standard. Silica gel 200–300 mesh (Qingdao Marine Chemical Inc., China) and
Sephadex LH-20 (Amersham Biosciences, Sweden) were used for column chromatography.
Medium pressure liquid chromatography (MPLC) was performed on a Büchi Sepacore
System equipping pump manager C-615, pump modules C-605 and fraction collector
C-660 (Büchi Labortechnik AG, Switzerland), and columns packed with Chromatorex
C-18 (40–75 μm, Fuji Silysia Chemical Ltd., Japan). Preparative HPLC was performed
on an Agilent 1260 liquid chromatography system equipped with a Zorbax SB-C18
column (5 μm, 9.4 mm × 150 mm).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!