Guanabenz acetate

Manufactured by Bio-Techne

Sourced in United Kingdom

Guanabenz acetate is a chemical compound used in research laboratory settings. It is a selective alpha-2 adrenergic agonist. The core function of Guanabenz acetate is to activate alpha-2 adrenergic receptors, which can be used to study related biological processes.

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Lab products found in correlation

3 protocols using guanabenz acetate

Chemical Treatments for In Vitro Experiments

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Tunicamycin (Calbiochem), Sodium-4-phenylbutyrate (EMD Millipore), Kifunensine (Tocris), Salubrinal (Calbiochem), Guanabenz acetate (Tocris) and GSK2606414 (EMD Millipore) were reconstituted in DMSO and diluted in MN media at the desired concentrations. Sodium-4-phenylbutyrate was used at 2 mM (Qi et al., 2004 (link)). For the other compounds, a dose response assay was performed to determine the most effective concentration for in vitro experiments (Figure S6).

Ng S.Y., Soh B.S., Rodriguez-Muela N., Hendrickson D.G., Price F., Rinn J.L, & Rubin L.L. (2015). Genome-Wide RNA-Seq of Human Motor Neurons Implicates Selective ER Stress Activation in Spinal Muscular Atrophy. Cell stem cell, 17(5), 569-584.

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Cell culture and treatments for insulinoma study

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The mouse
insulinoma cell
lines βTC6 (ATCC) and MIN6 were maintained in Dulbecco’s
modified Eagle’s medium (DMEM) supplemented with 15% fetal
bovine serum (FBS), 1× GlutaMAX, 1× nonessential amino acids,
and 1 mM sodium pyruvate. INS-1 rat insulinoma cells were maintained
in RPMI medium supplemented with 10% FBS, 1× GlutaMAX, 1×
nonessential amino acids, and 1 mM sodium pyruvate. Human islets were
obtained from the Integrated Islet Distribution Program (Duarte, CA)
in accordance with Oklahoma Medical Research Foundation internal review
board (IRB) and ethical guidelines for the use of human tissue. Standard
viability was 80–90% and purity was >80%. Islets were maintained
in CMRL medium supplemented with 10% FBS. All cells were grown at
37 °C in a humidified 5% CO₂ atmosphere. Tunicamycin
(Tm), brefeldin A (BFA), and thapsigargin (Tg) were from Sigma. CellTiter-Glo
and Caspase-Glo 3/7 reagents were from Promega. Salubrinal, guanabenz
acetate, STF083013, and kaempferol were from Tocris, quercetin and
apigenin were from Cayman, and PP1 Analog II was from Calbiochem.

Tran K., Li Y., Duan H., Arora D., Lim H.Y, & Wang W. (2014). Identification of Small Molecules That Protect Pancreatic β Cells against Endoplasmic Reticulum Stress-Induced Cell Death. ACS Chemical Biology, 9(12), 2796-2806.

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Pharmacological Agents for Neuroscience Research

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ARC-239, BRL-44408, clonidine hydrochloride, dexmedetomidine hydrochloride, efaroxan hydrochloride, guanabenz acetate, guanfacine hydrochloride, idazoxan hydrochloride, JP-1302, prazosin hydrochloride, rauwolscine hydrochloride, RS-79948, RX-821002, terazosin, UK-14304, WB-4101 and yohimbine hydrochloride were acquired from Tocris (Ellisville, MO). Atipamezole was from Orion Corporation (Espoo, Finland) and isoflurane was from Abbott Diagnostics (Chicago, IL). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).

Biggane J.P., Xu K., Goldenstein B.L., Davis K.L., Luger E.J., Davis B.A., Jurgens C.W., Perez D.M., Porter J.E, & Doze V.A. (2022). Pharmacological characterization of the α2A-adrenergic receptor inhibiting rat hippocampal CA3 epileptiform activity: Comparison of ligand efficacy and potency. Journal of receptor and signal transduction research, 42(6), 580-587.

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