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Hsc70

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Hsc70 is a molecular chaperone protein that plays a crucial role in protein folding, transport, and degradation processes within cells. It is a member of the heat shock protein 70 (Hsp70) family and is involved in maintaining the proper conformation and function of various cellular proteins.

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Lab products found in correlation

Gapdh, by Abcam (2 mentions) Lamp2a, by Thermo Fisher Scientific (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Ef 1α, by Cell Signaling Technology (1 mentions) Hsp90, by Cell Signaling Technology (1 mentions)

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Anti-Hsc70 (2 citations)

2 protocols using hsc70

1

Antibody Sources in Autophagy Assays

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Sources of reagents and chemicals were as described before (Kiffin et al., 2004; Massey et al., 2006; Bandyopadhyay et al., 2010). The antibody against mouse LAMP‐2A was developed in our laboratory (Cuervo & Dice, 1996). The antibody against total tau (DA9) was a gift from Dr. P. Davies (The Feinstein Institute, Manhasset, NY, USA). The antibody against Vps4 was from Sigma, against GAPDH from Abcam, against light chain 3 protein (LC3) and Atg7 from Cell Signaling (Danvers, MA, USA), against actin from Stressgen, and against hsc70 from Novus.
Caballero B., Wang Y., Diaz A., Tasset I., Juste Y.R., Stiller B., Mandelkow E., Mandelkow E, & Cuervo A.M. (2017). Interplay of pathogenic forms of human tau with different autophagic pathways. Aging Cell, 17(1), e12692.
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2

Antibody Sources for Cellular Analysis

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The sources of antibodies used in this study are LAMP-2A (Invitrogen); GAPDH (Abcam); RNase A (Rockland Immunochemicals); HSP90, β-actin, LC3B, and EF-1α (Cell Signaling Technology); hsc70 (Novus Biological); rHN (generated with residues 20–35 of the rHN peptide and no cross-reaction with HN and HNG; GeneTex); and mTOR CST and GFP antibody (Thermo Fisher Scientific). Antibody against HN or HNG was generated by Genscript using residues 7–24 of the HN peptide.
Gong Z., Tasset I., Diaz A., Anguiano J., Tas E., Cui L., Kuliawat R., Liu H., Kühn B., Cuervo A.M, & Muzumdar R. (2018). Humanin is an endogenous activator of chaperone-mediated autophagy. The Journal of Cell Biology, 217(2), 635-647.
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