Abbit anti cldn1

Rabbit anti-CLDN1, mouse anti-CLDN4, and rabbit anti-ZO-1 antibodies were obtained from Thermo Fisher Scientific (San Diego, CA, USA). Goat anti-β-actin and mouse anti-FLAG antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and Wako Pure Chemical (Osaka, Japan), respectively. Mouse anti-p-Ser and anti-p-Thr antibodies were from Sigma-Aldrich (Saint Louis, MO, USA). EBGP, LY, H₂DCFDA, and PMA were from Yamada Bee Company, Inc. (Lot: LY-009, Okayama, Japan), Biotium (Fremont, CA, USA), Thermo Fisher Scientific, and LC Laboratories (Woburn, MA, USA), respectively. OxiOrange and Hydrop were from Goryo Chemical (Hokkaido, Japan). All other reagents were of the highest grade of purity available.

Rabbit anti-CLDN1 and Alexa Fluor 555 anti-rabbit antibodies were obtained from Thermo Fisher Scientific (San Diego, CA, USA). Goat anti-β-actin antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). L-NAME, LY, NiSPY-3, Quest Fluo-8 AM, rabbit anti-nitrotyrosine, and rabbit anti-NOS3 antibodies were from Dojindo Laboratories (Kumamoto, Japan), Biotium (Fremont, CA, USA), Goryo Kagaku (Hokkaido, Japan), AAT Bioquest (Sunnyvale, CA, USA), R&D Systems (Minneapolis, MN, USA), and Cell Signaling Technology (Beverly, MA, USA), respectively. DAF-2DA, CAPE, apigenin, kaempferol, kaempferide, chrysin, quercetin, and apiin were from Cayman Chemical (Ann Arbor, MI, USA). EBGP was kindly provided from Yamada Bee Company (Okayama, Japan).