Anti human cd90 apc

For cell surface marker analysis, the confluent hDPSCs and pDPSCs were trypsinized and washed with PBS twice. The cells were then incubated for 30 min at 4 °C with antihuman-CD73-APC, anti-human-CD90-APC, anti-human-CD105-APC, antihuman-CD34-PE, antihuman-CD45-FITC and anti-human-HLA-DR-APC antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany). Antibody-stained cells were washed twice with PBS. 10,000 cells from each sample were acquired on Attune NxT Flow Cytometer (Thermo Fisher Scientific, Waltham, MA, USA). Isotype controls were used for the detection and to differentiate between positive and negative signals.

Flow cytometry is a biophysical technology used in cell counting, sorting and biomarker detection by measuring optical and fluorescence characteristics of single cells. Trypsinization was used to collect confluent SHEDs, which were then rinsed twice with PBS. Anti-human-CD29-PE, anti-human-CD90-APC, anti-human-CD73-APC, anti-human-CD105-APC, anti-human-CD140b-PE, anti-human-CD271-FITC, anti-human-CD34-PE, anti-human-CD45-FITC, anti-human-STRO-1-APC, and anti-human-HLA-DR-APC antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) were then incubated for 30 min at 4 °C. Antibody-labelled cells were given a wash twice in PBS beforehand being acquired on the low cytometry system at a rate of 10,000 cells per sample (Attune, Thermo Fisher Scientific, Waltham, MA, USA). To identify and distinguish between negative or positive signals, isotype controls were used.

Confluent DPSCs were harvested with trypsinization and washed twice with PBS for a cell surface marker analysis. Anti-human-CD73-APC, anti-human-CD90-APC, anti-human-CD105-APC, anti-human-CD34-PE, anti-human-CD45-FITC, and anti-human-HLA-DR-APC antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) were then added to the cells and incubated for 30 min at 4 °C. Antibody-labeled cells were washed twice in PBS before being counted on an Attune NxT flow cytometer (Thermo Fisher Scientific, Waltham, MA, USA) at a rate of 20,000 cells per sample. To identify and distinguish between positive and negative signals, isotype controls were used.

For cell surface marker analysis, DPSCs (Confluent) were collected by trypsinization and washed twice with Phosphate Buffered Saline. The cells were then incubated at 4 °C for 30 min with anti-human-CD73-APC, anti-human-CD90-APC, anti-human-CD105-APC, anti-human-CD34-PE, anti-human-CD45-FITC, and anti-human-HLA-DR-APC antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany). Antibody-stained cells were washed twice with PBS. A total of 10,000 cells per sample were acquired on Attune NxT Flow Cytometer (Thermo Fisher Scientific, Waltham, MA, USA). Isotype controls were used for the detection of and to differentiate between positive and negative signals.