Centrifugal filter tubes amicon 30 kda

Manufactured by Merck Group

Centrifugal filter tubes (Amicon-30 kDa) are a type of laboratory equipment used for separating and concentrating macromolecules such as proteins, nucleic acids, or other biomolecules based on their molecular weight. The tubes feature a semi-permeable membrane with a molecular weight cutoff of 30 kDa, allowing the passage of smaller molecules while retaining larger ones during centrifugation.

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Lab products found in correlation

Ammonium sulfate, by Merck Group (2 mentions) Maleimidocaproyl valine citrulline monomethyl auristatin e vcmmae, by MedChemExpress (2 mentions) Tween 20, by Merck Group (2 mentions) N acetylcysteine, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Isopropyl alcohol, by Merck Group (2 mentions) Kadcyla, by Roche (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) 2 mercaptoethanol, by Merck Group (1 mentions)

2 protocols using centrifugal filter tubes amicon 30 kda

Assay for ADC Characterization

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Ammonium sulfate, sodium phosphate, sodium chloride, Tris (2-carboxyethyl) phosphine (TCEP), N-acetylcysteine (NAC), isopropyl alcohol (IPA), dimethyl sulfoxide (DMSO), 2-mercaptoethanol (2-ME), ethylenediaminetetraacetic acid (EDTA), phosphate buffered saline (PBS), and TWEEN^® 20 were purchased from Sigma-Aldrich. Centrifugal filter tubes (Amicon-30 kDa) were purchased from Merck Millipore. Maleimidocaproyl-valine-citrulline-monomethyl auristatin E (VCMMAE) was obtained from MedChem Express. Lithium Dodecyl Sulfate (LDS) sample loading buffer (4X) and 12% acrylamide of PAGE gel were purchased from Thermo Fisher Scientific. Grener F 96-well immunoplates were purchased from Sigma-Aldrich.

Chiang Z.C., Fang S., Shen Y.K., Cui D., Weng H., Wang D., Zhao Y., Lin J, & Chen Q. (2022). Development of Novel CD47-Specific ADCs Possessing High Potency Against Non-Small Cell Lung Cancer in vitro and in vivo. Frontiers in Oncology, 12, 857927.

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Analytical Workflow for Antibody-Drug Conjugates

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Ammonium sulfate, sodium phosphate, sodium chloride, tris(2-carboxyethyl) phosphine (TCEP), N-acetylcysteine (NAC), isopropyl alcohol (IPA), dimethyl sulfoxide (DMSO), 2-mercaptoethanol (2-ME), ethylenediaminetetraacetic acid (EDTA), phosphate-buffered saline (PBS), and TWEEN^® 20 were purchased from Sigma-Aldrich. Centrifugal filter tubes (Amicon-30 kDa) were purchased from Merck Millipore. N-Succinimidyl-4-(maleimidomethyl) cyclohexanecarboxylate-emtansine (SMCC-DM1) was obtained from ALB Technology. Kadcyla^® was purchased from Roche. Maleimidocaproyl-valine-citrulline-monomethyl auristatin E (VCMMAE) was obtained from MedChem Express. Lithium dodecyl sulfate (LDS) sample loading buffer (4X) and 12% acrylamide PAGE gel were purchased from Invitrogen. NUNC 96-well Maxisorb immunoplates were purchased from Thermo Fisher Scientific.

Chiang Z.C., Chiu Y.K., Lee C.C., Hsu N.S., Tsou Y.L., Chen H.S., Hsu H.R., Yang T.J., Yang A.S, & Wang A.H. (2020). Preparation and characterization of antibody-drug conjugates acting on HER2-positive cancer cells. PLoS ONE, 15(9), e0239813.

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