Sod typed assay kit hydroxylamine method

The MnSOD activity of mouse heart homogenates was measured using a superoxide dismutase (SOD) typed assay kit (hydroxylamine method) (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instructions.

To assess the oxidative stress in intracellular and periodontal tissues, the levels of ROS, malondialdehyde (MDA), and superoxide dismutase (SOD) were detected.
The total intracellular ROS levels were determined with the ROS Assay Kit (Beyotime, China). Briefly, cells were incubated with DCFH-DA reagent for 20 min at 37°C after treatment. The intracellular fluorescence intensity was then examined under a fluorescent inverted microscope (Olympus, Japan).
Mitochondrial-derived ROS levels in periodontal tissue were measured using the Mito SOX Red kit (Thermo Fisher Scientific, United States). The Mito SOX red reagent can be oxidized by superoxide to show red fluorescence and thus assess the ROS content. The staining process was carried out as directed. The Mito SOX red fluorescence of the sections was detected by confocal microscopy.
The SOD typed assay kit (Hydroxylamine method) and MDA assay kit (TBA method) (Nanjing Jiancheng Bioengineering Institute, China) were used to detect intracellular and serum levels of MDA and SOD activity. The experimental procedures were carried out strictly according to the instructions.