HEK293T and U87 cells were maintained in DMEM (Gibco) supplemented with 10% foetal bovine serum (FBS, LabTech) and 100 U/ml penicillin plus 100 μg/ml streptomycin (Pen/Strep; Gibco). THP‐1 cells were maintained in RPMI (Gibco) supplemented with 10% FBS and Pen/Strep. THP‐1‐IFIT‐1 cells that had been modified to express Gaussia luciferase under the control of the IFIT‐1 promoter were described previously (Mankan et al, 2014 ). THP‐1 Dual Control and cGAS−/− cells were obtained from Invivogen. Lopinavir (LPV), darunavir (DRV), nevirapine (NVP) and raltegravir were obtained from AIDS reagents. STING inhibitor H151 was obtained from Invivogen. JAK inhibitor ruxolitinib was obtained from CELL guidance systems. PF‐74 was obtained from Sigma. Lipopolysaccharide, IFNβ and poly I:C were obtained from PeproTech. Sendai virus was obtained from Charles River Laboratories. Herring testis DNA was obtained from Sigma. cGAMP was obtained from Invivogen. Anti‐IFNα/β receptor and control IgG2A antibodies were obtained from PBL Interferon Source and R&D systems, respectively. For stimulation of cells by transfection, transfection mixes were prepared using lipofectamine 2000 according to the manufacturer's instructions (Invitrogen).
Thp 1 dual control and cgas cells
Manufactured by InvivoGen
THP-1 Dual Control and cGAS−/− cells are laboratory cell lines used in research. THP-1 Dual Control cells are a variant of the THP-1 monocytic cell line that express a reporter gene for monitoring cellular activation. The cGAS−/− cells are a genetically modified version of the THP-1 cell line with the cGAS gene knocked out.
Automatically generated - may contain errors
Lab products found in correlation
Pen strep, by Thermo Fisher Scientific (3 mentions)
Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (3 mentions)
Lipopolysaccharide, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by EQT (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Herring testis dna, by Merck Group (2 mentions)
Pen strep, by Merck Group (2 mentions)
Opti mem, by Thermo Fisher Scientific (2 mentions)
Hepes, by Merck Group (2 mentions)
Hygromycin, by Thermo Fisher Scientific (2 mentions)
Puromycin, by Merck Group (2 mentions)
Zeocin, by InvivoGen (2 mentions)
Blasticidin, by InvivoGen (2 mentions)
Sting inhibitor h151, by InvivoGen (1 mentions)
Ruxolitinib, by Cell Guidance Systems (1 mentions)
Sendai virus, by Charles River Laboratories (1 mentions)
Cgamp, by InvivoGen (1 mentions)
Poly 1 c, by Thermo Fisher Scientific (1 mentions)
Herring testes ht dna, by Merck Group (1 mentions)
3 protocols using thp 1 dual control and cgas cells
1
Immune Response Pathway Modulation
2
Maintenance and Stimulation of Cell Lines
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HEK293T and U87 cells were maintained in DMEM medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Labtech) and 100 U ml−1 penicillin plus 100 μg ml−1 streptomycin (Pen/Strep; Gibco). THP-1-IFIT1 cells that had been modified to express Gaussia luciferase under the control of the IFIT1 promoter were described previously62 (link). THP-1 dual control and cGAS−/− cells were obtained from Invivogen. THP-1 IFIT1 cells were maintained in RPMI medium (Gibco) supplemented with 10% FBS and Pen/Strep. THP-1 dual cells were maintained in RPMI (Gibco) supplemented with 10% FBS, Pen/Strep, 25 mM HEPES (Sigma), 10 µg ml−1 of blasticidin (Invivogen) and 100 μg ml−1 of Zeocin (Invivogen). GHOST cells stably expressing CD4, CCR5, CXCR4 and the green fluorescent protein (GFP) reporter gene under the control of the HIV-2 long terminal repeat, were maintained in DMEM supplemented with 10% FBS, and antibiotics, G418 (500 μg ml−1) (Thermo Fisher), hygromycin (100 μg ml−1)(Invitrogen) and puromycin (1 μg ml−1) (Millipore). Lipopolysaccharide, IFNβ, IL-1β and poly I:C were obtained from Peprotech. Herring testes (HT) DNA was obtained from Sigma. For stimulation of cells by transfection, transfection mixes were prepared using lipofectamine 2000 (Invitrogen) in Optimem (Thermo Fisher). HT DNA and poly I:C concentration used are stated on each figure.
3
Cell Culture Conditions for Immune Assays
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HEK293T and U87 cells were maintained in DMEM (Gibco) supplemented with 10 % foetal bovine serum (FBS, Labtech) and 100 U/ml penicillin plus 100 μg/ml streptomycin (Pen/Strep; Gibco). THP-1-IFIT-1 cells that had been modified to express Gaussia luciferase under the control of the IFIT-1 promoter were described previously (Mankan et al., 2014) . THP-1 Dual Control and cGAS-/-cells were obtained from Invivogen. THP-1 IFIT1 cells were maintained in RPMI (Gibco) supplemented with 10 % FBS and Pen/Strep. THP-1 Dual cells were maintained in RPMI (Gibco) supplemented with 10 % FBS, Pen/Strep, 25 mM HEPES (sigma), 10 µg/ml of blasticidin (Invivogen) and 100 μg/ml of Zeocin™ (Invivogen). GHOST cells stably expressing CD4, CCR5, CXCR4, and the green fluorescent protein (GFP) reporter gene under the control of the HIV-2 long terminal repeat, were maintained in DMEM supplemented with 10% FBS, and antibiotics, G418 (500 μg/ml) (Thermo Fisher), hygromycin (100 μg/ml)(Invitrogen) and puromycin (1 μg/ml) (Millipore). Lipopolysaccharide, IFNβ, IL-1b and poly I:C were obtained from Peprotech. Herring-testis DNA was obtained from Sigma. For stimulation of cells by transfection, transfection mixes were prepared using lipofectamine 2000 in Optimem (Thermofisher Scientific) according to the manufacturer's instructions (Invitrogen).
HT DNA and poly I:C concentration used are stated on each figure.
HT DNA and poly I:C concentration used are stated on each figure.
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