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Mab ul44

Manufactured by Merck Group
Sourced in United States

MAb-UL44 is a laboratory equipment product developed by Merck Group. It is a monoclonal antibody that specifically binds to the UL44 protein, which is a component of the human cytomegalovirus (HCMV) DNA polymerase complex. The core function of MAb-UL44 is to facilitate the detection and identification of HCMV in research and diagnostic settings.

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2 protocols using mab ul44

1

Western Blot Analysis of HCMV Proteins

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SDS-PAGE and standard Western blot analysis, based on equalized amounts of the total cell lysates, were performed as described previously in [64 (link)]. The antibodies used for staining were mAb-HA (H9658, Sigma-Aldrich, USA), pAb-Flag (F7425, Sigma-Aldrich), mAb-IE1p72 (P63-27) [65 (link)], and mAb-pp28 (both kindly provided by William J. Britt, Dept. Pediatrics Microbiol., Univ. Alabama, Birmingham, AL, USA), mAb-UL44 (kindly provided by Bodo Plachter, Virology, Univ. Mainz, Germany), and mAb-actin (A5441, Sigma-Aldrich).
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2

Antibody Panel for Protein Analysis

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The following polyclonal (pAb) and monoclonal (mAb) antibodies were used: mAb-UL97 (clone 1C4/0.2, produced and kindly provided by Dr. T. Lenac/Prof. S. Jonjic, Dept. Histology and Embryology, Univ. Rijeka, Croatia), pAb-UL97 (06-09, kindly provided by Prof. D. M. Coen, Harvard Medical School, Boston, MA, USA), mAb-UL44 (BS 510, kindly provided by Prof. B. Plachter, Univ. Mainz, Mainz, Germany), mAb-pp65 (65-33, kindly provided by Prof. W. J. Britt, UAB, Birmingham, AL, USA), mAb-β-actin (AC-15, Sigma-Aldrich), mAb-cyclin B1 (sc-7393, Santa Cruz; GNS11, Thermo Fisher Scientific, Waltham, MA, USA), pAb-cyclin B1 (sc-752, Santa Cruz), mAb-cyclin T1 (sc-271348, Santa Cruz), pAb-cyclin T1 (sc-10750, Santa Cruz), pAb-Fc (rabbit Fc fragment, Jackson ImmunoResearch Laboratories, Bar Harbor, ME, USA), mAb-Flag (M2, Sigma-Aldrich), pAb-Flag (F7425, Sigma Aldrich), and mAb-HA (Clone 7, Sigma-Aldrich). The following fluorescent dye-conjugated secondary antibodies were applied in immunofluorescence analyses: Alexa 488-conjugated goat anti-rabbit IgG (H+L) and Alexa 555-conjugated goat anti-mouse IgG (H+L) (Life Technologies, Carlsbad, CA, USA).
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