Nimbolide

Manufactured by Abcam

Sourced in United States

Nimbolide is a natural compound extracted from the neem tree (Azadirachta indica). It is a laboratory reagent that can be used for research purposes. Nimbolide has been studied for its potential biological activities, but its core function as a research tool is to provide a compound for scientific investigation without any interpretation or extrapolation on intended use.

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Lab products found in correlation

Luteolin, by Merck Group (1 mentions) Gedunin, by Bio-Techne (1 mentions) Enoxacin, by Merck Group (1 mentions) Sybr green 1 nucleic acid stain, by Lonza (1 mentions) Pyronaridine tetraphosphate, by Merck Group (1 mentions)

2 protocols using nimbolide

HPLC-MS/MS Protocol for Natural Compounds

Cited 1 time

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The fractions collected from HPLC were separated on a reversed-phase analytical column (2.1 × 30 mm, 3.5 μm; Zorbax Eclipse 300SB-C18;) on a MSD-TOF instrument (Agilent Technologies) in a positive-mode ESI, as described in ref. 21 (link). The instrument was operated with mobile phase A as water and phase B as methanol, with a gradient 50% B to 100% B over 10 minutes, 100% B for 5 minutes and 5 minutes to equilibrate, and a flow rate of 300 μL/min. The scan range for acquisition was 300 to 1,000 counts versus mass-to-charge (m/z) range; scan rate: 1 spectra/s; gas temperature 325°C; gas flow 7 L/min; Nebulizer: 40 psi; Capillary voltage: 3,800 V; Fragmentor: 140 V; Skimmer: 65 V; Octapol: 750 V. Nimbolide, purchased from BioVision Inc., and 2′,3′-dehydrosalannol, purchased from Sigma-Aldrich, were used as standards.

Wu Q., Kohli M., Bergen HR I.I.I., Cheville J.C., Karnes R.J., Cao H., Young C.Y., Tindall D.J., McNiven M.A, & Donkena K.V. (2014). Preclinical Evaluation of the Supercritical Extract of Azadirachta Indica (Neem) Leaves In Vitro and In Vivo on Inhibition of Prostate Cancer Tumor Growth. Molecular cancer therapeutics, 13(5), 1067-1077.

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Nucleic Acid Staining and Compound Preparation

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SYBR Green I (SGI) nucleic acid stain (Lonza, USA; 10,000x) was stored at -20°C and thawed before use. A lysis buffer consisting of Tris (130 mM; pH 7.5), EDTA (10 mM), saponin (0.016%; W/V), and TritonX-100 (1.6%; V/V) was prepared in advance and stored at 4°C. Diminazene aceturate (Novartis, Japan), luteolin (Sigma-Aldrich, Japan), pyronaridine tetraphosphate (Sigma-Aldrich), nimbolide (BioVision, USA), gedunin (Tocris Bioscience, UK), and enoxacin (Sigma-Aldrich) were prepared as 100 mM or 50 mM stock solutions.

Rizk M.A., El-Sayed S.A., Terkawi M.A., Youssef M.A., El Said E.S., Elsayed G., El-Khodery S., El-Ashker M., Elsify A., Omar M., Salama A., Yokoyama N, & Igarashi I. (2015). Optimization of a Fluorescence-Based Assay for Large-Scale Drug Screening against Babesia and Theileria Parasites. PLoS ONE, 10(4), e0125276.

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