Alexafluor goat anti mouse 488 secondary antibody

Manufactured by Thermo Fisher Scientific

The Alexafluor goat anti-mouse 488 secondary antibody is a fluorescently labeled antibody used in immunoassays and cell-based applications to detect and visualize mouse primary antibodies. The antibody is conjugated with the Alexa Fluor 488 dye, which emits green fluorescence upon excitation, allowing for sensitive detection of target proteins or cellular structures.

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Lab products found in correlation

Prolong diamond antifade, by Thermo Fisher Scientific (2 mentions) Formaldehyde, by Polysciences (1 mentions)

Close spelling variants of this product

Goat anti-mouse-Alexafluor 488 conjugated secondary antibody Secondary goat anti-mouse antibody coupled to AlexaFluor 488 AlexaFluor 488-labelled goat anti-mouse secondary antibody Anti Alexafluor-488 antibody Goat anti-mouse secondary antibody Secondary anti-mouse antibody AlexaFluor antibody

2 protocols using alexafluor goat anti mouse 488 secondary antibody

Immunofluorescence Staining of EphA2

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Example 11

In chamber slides, cells were plated and allowed to adhere overnight in humidified cell culture incubators. The following day, cells were treated with the indicated ligands for 30 min. Subsequently, cells were washed with PBS and fixed with 4% Formaldehyde (Polysciences, Inc.), then blocked and permeabilized with 5% FBS and 0.3% Triton™ X-100 for 1 h. Next, wells were washed and stained with anti-EphA2 antibody, followed by three washes and further incubation with Alexafluor goat anti-mouse 488 secondary antibody (ThermoFisher). Cells were counterstained with DAPI and mounted with Prolong Diamond Antifade (ThermoFisher). Images were acquired with confocal microscope Zeiss 880 Airyscan and processed using Adobe Photoshop CC.

US11739121B2. EPHA2 agonists and uses thereof (2023-08-29). THE REGENTS OF THE UNIVERSITY OF CALIFORNIA [US]. Inventors: Maurizio Pellecchia [US], Luca Gambini [US], Alexander Aronson [US].

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Visualizing EphA2 Receptor Localization

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In chamber slides, cells were plated and allowed to adhere overnight in humidified cell culture incubators. The following day, cells were treated with the indicated ligands for 30 min. Subsequently, cells were washed with PBS and fixed with 4% formaldehyde (Polysciences, Inc.), then blocked and permeabilized with 5% FBS and 0.3% Triton X-100 for 1 h. Next, wells were washed and stained with anti-EphA2 antibody, followed by three washes and further incubation with Alexafluor goat antimouse 488 secondary antibody (ThermoFisher). Cells were counterstained with DAPI and mounted with Prolong Diamond Antifade (ThermoFisher). Images were acquired with confocal microscope Zeiss 880 Airyscan and processed using Adobe Photoshop CC.

Gambini L., Salem A.F., Udompholkul P., Tan X.F., Baggio C., Shah N., Aronson A., Song J, & Pellecchia M. (2018). Structure-Based Design of Novel EphA2 Agonistic Agents with Nanomolar Affinity in Vitro and in Cell. ACS chemical biology, 13(9), 2633-2644.

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