Anti cd117

Manufactured by Miltenyi Biotec

Sourced in Germany, Italy

Anti-CD117 is a monoclonal antibody that binds to the CD117 antigen, also known as c-kit. CD117 is a receptor tyrosine kinase that plays a role in the regulation of cell survival, proliferation, and differentiation. The core function of Anti-CD117 is to facilitate the detection and analysis of CD117-expressing cells.

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Lab products found in correlation

Kaluza acquisition software, by Beckman Coulter (1 mentions) Anti sca 1, by Miltenyi Biotec (1 mentions) Anti cd31 antibody, by Miltenyi Biotec (1 mentions) Gallios flow cytometer, by Beckman Coulter (1 mentions) Fcs express 4, by De Novo Software (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) 4 oh tamoxifen, by Merck Group (1 mentions) Magnetic cell sorting separator, by Miltenyi Biotec (1 mentions)

Close spelling variants of this product

Anti-c/kit (CD117) antibody Anti-CD117/c-Kit microbeads Anti-CD117 PE (c-kit) Anti-CD117 magnetic beads Anti-CD117 microbeads Anti-CD117 immunomagnetic beads Anti-CD117 magnetic microbeads Anti-mouse CD117 MicroBeads Anti-CD117-APC Anti-CD117 beads

3 protocols using anti cd117

Phenotypic Analysis of Cultured hCSCs

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Cultivated hCSCs were harvested by centrifugation after treatment with trypsin and subsequently stained with PE-coupled anti-CD105, anti-CD117, anti-Sca1 or anti-CD31 antibody (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s guidelines. For isotype controls, hCSCs were stained with PE-coupled IgG1 control antibody or APC-coupled IgG1 control antibody. Analysis was done using Gallios Flow Cytometer (Beckmann Coulter Inc., Brea, CA, USA), while Kaluza Acquisition Software (Beckmann Coulter Inc.) was used for subsequent data acquisition and statistical analysis.

Höving A.L., Schmidt K.E., Merten M., Hamidi J., Rott A.K., Faust I., Greiner J.F., Gummert J., Kaltschmidt B., Kaltschmidt C, & Knabbe C. (2020). Blood Serum Stimulates p38-Mediated Proliferation and Changes in Global Gene Expression of Adult Human Cardiac Stem Cells. Cells, 9(6), 1472.

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Flow Cytometry Analysis of Transfection Efficiency

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Flow cytometry was used to detect cells transfected with p-IRES-AcGFP and p-IRES-AcGFP-c-KIT plasmids using CyFlow® Cube 8 Sorter Flow Cytometer (Sysmex Partec, Gorlitz, Germany) and evaluate transfection efficiency.
2x10⁷ cells per sample were washed with PBS and incubated with PE conjugated monoclonal mouse antibody anti-CD117 (Miltenyi Biotec Cat# 130-091-734, RRID:AB_615058) (Miltenyi Biotec, Calderara di Reno, BO, Italy) following the manufacturer’s instructions.
Each sample was analyzed in the same run, with identical settings equipped with a blue diode pumped solid-state Laser (20mV) at 488 nm. At least 10,000 events per samples were collected and the gating strategy was based on Forward Scatter (FSC) and Side Scatter (SSC) characteristics. Data analysis was performed using FCS express 4 software (De Novo Software™).

Franceschi S., Lessi F., Panebianco F., Tantillo E., La Ferla M., Menicagli M., Aretini P., Apollo A., Naccarato A.G., Marchetti I, & Mazzanti C.M. (2017). Loss of c-KIT expression in thyroid cancer cells. PLoS ONE, 12(3), e0173913.

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Spermatogonia Enrichment Protocol

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Spermatogonia were enriched by selection of C-kit+ or Gfra1+ cells. C-kit+ (CD117) cells were isolated from whole tubule cell suspensions on a magnetic cell-sorting separator (Miltenyi Biotec) using an anti-CD117 (Miltenyl Biotec) antibody. Cells were additionally stained with a biotinylated anti-CD117 antibody (1:200) for 20 min followed by streptavidin conjugated Alexa Fluor 488 (1:1000, Life Technologies) for 20 min prior to flow cytometry. For Gfra1+ selection, tamoxifen inducible Gfrα1^CreERT2 mice on a C57BL/6 background (kindly provided by Dr. Shosei Yoshida, National Institute for Basic Biology, Okazaki, Japan) were crossed with B6.Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo} (Rosa^mT/mG; JAX®mice, stock no. 007676). Labeling of Gfra1+ spermatogonia was conducted by injecting a Gfrα1^CreERT2; Rosa^mT/mG mouse with 2mg of 4OH-tamoxifen (dissolved in ethanol and then in corn oil, Sigma) per day for 3 weeks prior to euthanasia at 8 weeks of age. All live Gfra1 positive (GFP expressing) and tdTomato negative cells were collected by flow cytometry. The extended labeling was necessary to obtain the number of cells needed for Drop-seq. As a result this dataset includes spermatogonia and spermatocytes.

Green C.D., Ma Q., Manske G.L., Shami A.N., Zheng X., Marini S., Moritiz L., Sultan C., Gurczynski S.J., Moore B.B., Tallquist M.D., Li J.Z, & Hammoud S.S. (2018). A comprehensive roadmap of murine spermatogenesis defined by single-cell RNA-seq. Developmental cell, 46(5), 651-667.e10.

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