Human keratinocyte HaCaT cells were obtained from the Cell Lines Service (Eppelheim, Germany). Human primary neonatal keratinocyte HEKn cells were purchased from ThermoFisher Scientific Korea (Seoul, Korea). HaCaT cells were maintained in DMEM medium (1.8 mM CaCl2) supplemented with 10% fetal bovine serum (HyClone, Logan, UT, USA) and HEKn cells were cultured in EpiLife medium supplemented with Human Keratinocyte Growth Supplement (HKGS) (ThermoFisher Scientific Korea). Agerarin was isolated as described previously [15 (link)]. 2,4-Dinitrochlorobenzene (DNCB) was obtained from Sigma–Aldrich (St. Louis, MO, USA). TNFα and IFNγ were obtained from Prospec-Tany Technogene Ltd. (Ness-Ziona, Israel). The hematoxylin and eosin (H&E) stain kit and toluidine blue dye were obtained from Sigma–Aldrich. The Firefly Luciferase Assay System was from Promega (Madison, WI, USA). Antibodies against phospho-JAK1 (Y1034/1035), JAK1, phospho-JAK2 (Y1007/1008), JAK2, phospho-STAT3 (Y705), and STAT3 were purchased from Cell Signaling Technology (Beverly, MA, USA).Antibodies against FLG and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-FLG antibody for immunofluorescent staining was from BioLegend (San Diego, CA, USA).
Hekn cells
Manufactured by Thermo Fisher Scientific
HEKn cells are a cell line derived from human embryonic kidney cells. They are commonly used in cell biology research and serve as a model system for studying various cellular processes and functions.
Automatically generated - may contain errors
Lab products found in correlation
Epilife medium, by Thermo Fisher Scientific (2 mentions)
Toluidine blue dye, by Merck Group (1 mentions)
Hacat cell, by Cytion (1 mentions)
Firefly luciferase assay system, by Promega (1 mentions)
Phospho stat3 y705, by Cell Signaling Technology (1 mentions)
2 4 dinitrochlorobenzene dncb, by Merck Group (1 mentions)
Stat3, by Cell Signaling Technology (1 mentions)
Tnf α, by ProSpec (1 mentions)
Ifn γ, by ProSpec (1 mentions)
Hela cell, by Thermo Fisher Scientific (1 mentions)
Hela cell, by American Type Culture Collection (1 mentions)
2 protocols using hekn cells
1
Keratinocyte Activation and Inflammatory Regulation
2
Culturing Human Cancer and Skin Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HeLa cells (human cervical cancer cell line) (American Type Culture Collection, Manassas, VA, USA) were cultured in supplemented RPMI-1640 with fetal bovine serum (10 % V/V), penicillin-streptomycin (100 UI/mL and 100 µg/mL, respectively) and 2 mM glutamine, and incubated at normal culturing conditions (37 °C, 5% CO 2 ). Fresh medium was replaced every 2 days up to adequate confluency for subcultivation.
HEKn cells (epidermal keratinocytes isolated from neonatal foreskin) (Thermo Fisher Scientific) were cultivated in EpiLife® Medium supplemented with 60 µM calcium, an antibiotic/antimycotic solution of gentamicin and amphotericin B and an human keratinocyte growth supplement (1% V/V; composed by bovine pituitary extract (0.2% V/V), recombinant human insulin-like growth factor-I (1 µg/mL), hydrocortisone (0.18 µg/mL), bovine transferrin
(5 µg/mL) and human epidermal growth factor (0.2 ng/mL)) and an antibiotic/antimycotic solution of gentamicin and amphotericin B, incubated and maintained in the conditions of HeLa cells.
HEKn cells (epidermal keratinocytes isolated from neonatal foreskin) (Thermo Fisher Scientific) were cultivated in EpiLife® Medium supplemented with 60 µM calcium, an antibiotic/antimycotic solution of gentamicin and amphotericin B and an human keratinocyte growth supplement (1% V/V; composed by bovine pituitary extract (0.2% V/V), recombinant human insulin-like growth factor-I (1 µg/mL), hydrocortisone (0.18 µg/mL), bovine transferrin
(5 µg/mL) and human epidermal growth factor (0.2 ng/mL)) and an antibiotic/antimycotic solution of gentamicin and amphotericin B, incubated and maintained in the conditions of HeLa cells.
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