The bacterial enumerations in the probiotic whey beverages were performed using the pour plate technique. Streptococcus salivarius subsp. Thermophilus (S. thermophilus) was enumerated using M17 Agar (Merck, Darmstadt, Germany) and Lactobacillus acidophilus was enumerated using MRS-Sorbitol Agar (Oxoid Ltd., Hampshire, UK) according to Dave and Shah [31 (link)]. TOS-MUP Agar (Merck, Darmstadt, Germany) was used for Bifidobacterium animalis subsp. Lactis (Bb-12) enumeration according to Raeisi et al. [32 (link)]. Anaerobic jars (Aaerocult A, Merck, Darmstadt, Germany) were used for the incubation of bacteria, except S. thermophilus which was incubated under aerobic conditions. The incubation temperature for S. thermophilus, L. acidophilus, and Bb-12 was 37 °C for 48 h, 72 h, and 72 h, respectively. Cell counts were expressed as log cfug−1.
Tos mup agar
Manufactured by Merck Group
Sourced in Germany
TOS-MUP Agar is a microbiological culture medium used for the detection and enumeration of microorganisms. It is designed to support the growth of a wide range of bacteria, including coliforms and Escherichia coli (E. coli). The medium contains tryptone, soy peptone, and selective agents that inhibit the growth of non-target microorganisms, allowing for the specific identification and quantification of the desired bacterial species.
Automatically generated - may contain errors
Lab products found in correlation
M17 agar, by Merck Group (1 mentions)
S sobrinus, by American Type Culture Collection (1 mentions)
S mutans, by American Type Culture Collection (1 mentions)
L acidophilus, by American Type Culture Collection (1 mentions)
L casei, by American Type Culture Collection (1 mentions)
B lactis, by American Type Culture Collection (1 mentions)
B longum, by American Type Culture Collection (1 mentions)
Rogosa agar, by BD (1 mentions)
2 protocols using tos mup agar
1
Enumeration of Probiotic Bacteria in Whey Beverages
2
Evaluation of Probiotic Bacterial Strains
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The bacterial species evaluated in this study were B. animalis (from ACTIVIA ® ), B. longum, (ATCC 15707), B. lactis (LMG 18905), and B. dentium (ATCC 27678); L. acidophilus (ATCC 4356), L. casei (ATCC 393); S. mutans (ATCC 25175 and 3VF2), and S. sobrinus (ATCC 27607). All ATCC strains were obtained from the Oswaldo Cruz Foundation (FIOCRUZ, Rio de Janeiro, RJ, Brazil), and the André Tosello Foundation (Campinas, SP, Brazil). S. mutans 3VF2 is a highly acidogenic clinical strain that was previously characterized and also provided by Dr. Renata de Oliveira Mattos-Graner (FOP-UNICAMP, Piracicaba, Brazil). 9 B. animalis was isolated from ACTIVIA ® yogurt. Bifidobacterium, Lactobacillus, and Streptococcus species were isolated from the following media: transgalactosylated oligosaccharides propionate agar supplemented with lithium mupirocin (50 mg/L) (TOS-MUP agar; Merck Millipore, Darmstadt, Germany), Rogosa Agar (Difco Laboratories, Detroit, USA) and Mitis Salivarius Agar (MSA, Difco), respectively. 7 (link) All bacteria were grown at 37 o C for 48 h in a 5% CO 2 atmosphere.
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