LC-MS grade methanol (MeOH) and acetonitrile (CH3CN) were used for the extraction and analyses of toxins by liquid chromatography coupled to mass spectrometry (LC-MS). Analytical-grade ammonium hydroxide, sodium hydroxide (NaOH), and hydrochloric acid (HCl) were used for the mobile phase and hydrolysis. Ultrapure water was obtained with a Sartorius (Arium Pro) purification system. Certified reference solutions for okadaic acid CRM-OA-d (batch #20141119), dinophysistoxin-2 CRM-DTX2-b (batch #20150819), dinophysistoxin-1 CRM-DTX1-b (batch #20151209), and pectenotoxin-2 CRM-PTX2-b (batch #20120516) were obtained from NRC-CNRC.
Arium pro purification system
Manufactured by Sartorius
The Arium Pro water purification system is designed to produce ultrapure water for laboratory applications. It utilizes a multi-stage filtration process to remove contaminants and deliver consistently high-quality water for critical experiments and analyses.
Automatically generated - may contain errors
3 protocols using arium pro purification system
1
Extraction and Analysis of Toxins by LC-MS
2
Canagliflozin Formulation: Improving Solubility
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Canagliflozin (hemihydrate, D90: 18.119 µm) was purchased from Fuxin Long Rui Pharmaceutical CO., Ltd. (Fuxin, China). Hydroxyl propyl methyl cellulose 15 cP (Methocel®E15 LV) was obtained from Colorcon Middle East Pvt. Lt. (Istanbul, Turkey). Soluplus®, poloxamer 188 (P188, Kolliphor® P188), poloxamer 407 (P407, Kolliphor® P407), sodium lauryl sulfate (SLS, Kolliphor® SLS fine), polyvinylpyrrolidone—polyvinyl acetate copolymers (PVP VA64, Kollidon® VA 64) and polyvinylpyrrolidone (PVP K30, Kollidon® 30) were obtained from BASF, Ludwigshafen, Germany. Polysorbate 80 (Tween® 80) and polysorbate 20 (Tween® 20) were taken from Sigma-Aldrich Chemie GmbH, Steinheim, Germany. Methanol, acetonitrile (HPLC grade) and 85% ortho-phosphoric acid were obtained from MERCK Millipore, Darmstadt, Germany and ISOLAB, Wertheim, Germany, respectively. Ultrapure water was prepared by using a Sartorius Arium Pro purification system.
3
Biophysical Characterization of Nucleic Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The aqueous buffer used was NaCl 0.1 M, NaCac 2.5 mM (Cac = cacodylate) pH = 7.0, with the exception of the melting experiment with CT-DNA where the buffer was NaCac 2.5 mM pH = 7.0. For the experiments with G4s the buffer was KCl 0.1M LiCac 2.5mM pH 7.0 for the titrations and melting analysis, while for the mass spectrometry the buffer was NH 4 OAc 0.1M pH 7.0. Ultra-pure water from an ultra-pure Sartorius Arium-pro purification system was used throughout. Common reagents were the commercial analytical grade compounds and were used as received.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!