2‐CTC resin, N‐α‐Fmoc‐L‐amino acids and building blocks used during chain assembly were purchased from Iris Biotech GmbH (Marktredwitz, Germany). Ethyl cyanoglyoxylate‐2‐oxime (Oxyma) was purchased from Novabiochem (Darmstadt, Germany), N,N′‐dimethylformamide (DMF) and trifluoroacetic acid (TFA) were from Carlo Erba (Rodano, Italy). N,N′‐diisopropylcarbodiimide (DIC), dichloromethane (DCM), and all other organic reagents and solvents, unless otherwise stated, were purchased in high purity from Sigma‐Aldrich (Steinheim, Germany). All solvents for solid‐phase peptide synthesis (SPPS) were used without further purification. HPLC grade acetonitrile (ACN) and ultrapure 18.2 Ω water (Millipore‐MilliQ) were used for the preparation of all buffers for liquid chromatography. The chromatographic columns were from Phenomenex (Torrance CA, USA). HPLC eluent A: 97.5 % H2O, 2.5 % ACN, 0.7 %TFA; HPLC eluent B: 30 % H2O, 70 % ACN, 0.7 % TFA.
N n dimethylformamide (dmf)
Manufactured by Carlo Erba
Sourced in Germany, France, Italy
N,N′‐dimethylformamide (DMF) is a clear, colorless liquid that is widely used as a solvent in various laboratory applications. It has a high boiling point and is miscible with water and many organic solvents. DMF is a polar aprotic solvent, making it a suitable choice for a range of chemical reactions and processes.
Automatically generated - may contain errors
Lab products found in correlation
N α fmoc l amino acids, by Iris Biotech (2 mentions)
Hplc grade acetonitrile acn, by Merck Group (1 mentions)
Humic acid, by Merck Group (1 mentions)
Chloroform, by Thermo Fisher Scientific (1 mentions)
Zrcl4, by Merck Group (1 mentions)
Terephthalic acid, by Thermo Fisher Scientific (1 mentions)
Dichloromethane dcm, by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Carlo Erba (1 mentions)
Milli q gradient a10 system, by Merck Group (1 mentions)
Fluorescein isothiocyanate (fitc), by Merck Group (1 mentions)
Diethyl ether, by Avantor (1 mentions)
N n diisopropylethylamine dipea piperidine, by Merck Group (1 mentions)
N methylmorpholine, by Merck Group (1 mentions)
4 protocols using n n dimethylformamide (dmf)
1
Solid-Phase Peptide Synthesis Protocol
2
Fabrication of Zr-based Hybrid Membranes
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ZrCl4 was purchased from MERCK Schuchardt Hamburg, Germany. Terephthalic acid (99+% pure, MW 166.13 g/mol) was purchased from ACROS Organics, Geel, Belgium. Methanol and Chloroform (analytical reagent grade) were bought from Fisher Scientific, Geel, Belgium. PSf polymer and humic acid were purchased from Sigma Aldrich, Hamburg, Germany. NMP (1-methyl-2-pyrrolidinone) and N, N-dimethylformamide (DMF) were obtained from CARLO ERBA Reagents (Le Vaudreuil, France). Zeolite 4A (Molecular Sieve 4A, Powder) was purchased from ACROS Organics, Geel, Belgium.
3
Peptide Synthesis Using Fmoc-Based SPPS
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All of the N-α-Fmoc-L-amino acids and chlorotrityl chloride resin (CTC) used for the peptide synthesis were purchased from Iris Biotech GmbH (Marktredwitz, Germany). Fmoc-PEG building block, 8-(9-Fluorenyloxycarbonyl-amino)-3,6-dioxaoctanoic acid (Fmoc-O2Oc-OH), oxyma pure, and diisopropyl carbodiimide (DIC) were also from Iris Biotech. Trifluoroacetic acid (TFA) and N,N′-dimethylformamide (DMF) were from Carlo Erba (Rodano, Italy). Dichloromethane (DCM), N,N′-diisopropylethylamine (DIEA), phosphatidylcholine (PC), 10,12-tricosadiynoic acid (polydiacetylene, PDA), and all other organic reagents and solvents were purchased in high purity from Merck KGaA (Darmstadt, Germany), unless stated otherwise. All of the solvents for the solid-phase peptide synthesis (SPPS) were used without further purification. For the preparation of all of the solvents for liquid chromatography, HPLC-grade acetonitrile and ultrapure 18.2 Ω water (MilliQ) were used.
4
Solid-Phase Peptide Synthesis Reagents
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Polypropylene reaction vessels and sintered polyethylene frits were supplied by Alltech Italia (Milan, Italy). NovaSyn TGR resin, 2-(1Hbenzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU), cyano-hydroxyimino-acetic acid ethyl ester (Oxyma) and all amino acids were purchased from Novabiochem-Merck (Nottingham, U.K.). N,N′-diisopropylethylamine (DIPEA), piperidine, Kaiser test, trifluoroacetic acid (TFA), scavengers, fluorescein isothiocyanate (FITC) and N-methylmorpholine (NMM) were purchased from Sigma-Aldrich (Milan, Italy). N,N-Dimethylformamide (DMF) was purchased from CARLO ERBA Reagents (Milan, Italy). Acetonitrile (ACN), dichloromethane (DCM) and diethyl ether were purchased from VWR International (Milan, Italy). All aqueous solutions were prepared by using water obtained from a Milli-Q gradient A-10 system (Millipore, 18.2 MΩ•cm, organic carbon content ≥ 4 μg/L).
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