Dfc 3200 camera

Manufactured by Leica camera

Sourced in Japan

The Leica DFC 3200 is a high-resolution digital camera designed for use in laboratory and scientific imaging applications. It features a 3.2-megapixel sensor and captures images with a resolution of 2048 x 1536 pixels. The camera is capable of capturing both monochrome and color images, and provides a range of exposure and imaging settings to accommodate various experimental requirements.

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Lab products found in correlation

Ckx41 microscope, by Leica camera (3 mentions) Ckx41 microscope, by Olympus (2 mentions) Ab56023, by Abcam (2 mentions) Ab150077, by Abcam (2 mentions) Fluorescently labeled secondary antibody, by Thermo Fisher Scientific (1 mentions) Chondroitinase, by Merck Group (1 mentions) Cryotome, by Leica camera (1 mentions) Horse serum, by Vector Laboratories (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Hyaluronidase, by Merck Group (1 mentions) 5 ethynyl 2 deoxyuridine edu, by Thermo Fisher Scientific (1 mentions) 5 ethynyl 2 deoxyuridine (edu), by Thermo Fisher Scientific (1 mentions) Bz x800 microscope, by Keyence (1 mentions) Ab53444, by Abcam (1 mentions) Ab150158, by Abcam (1 mentions) Ix81 inverted microscope, by Olympus (1 mentions) Colorfrost plus microscope slides, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole dapi containing mounting medium, by Vector Laboratories (1 mentions) Metamorph software, by Molecular Devices (1 mentions)

5 protocols using dfc 3200 camera

Histological Analysis of Cartilage Tissue

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Following fixation, the samples were washed with PBS (Life Technologies), decalcified for 4 days in Versenate EDTA solution (American Master Tech). Processed samples were then taken through a sucrose gradient (10, 20, 30%), embedded in OCT compound (Tissue-Tek), sectioned (16 µm thick) on a cryotome (Leica), and mounted on glass slides (n = 4 section per sample). Antigen retrieval was performed with 1 mg/ml chondroitinase (Sigma-Aldrich) and 5 mg/ml hyaluronidase (Sigma-Aldrich) for 30 min at 37°C. Nonspecific binding was suppressed with 1% horse serum (Vector Labs) in PBS for 45 min. Slides were then washed with 0.1% Triton X-100/TBS, blocked in 1% BSA, incubated with primary antibodies against collagen type II (Col2) (Abcam), myosin heavy chain (MHC) (Developmental Studies Hybridoma Bank), and/or proliferating cell nuclear antigen (PCNA) (Abcam) overnight at 4°C, and incubated with fluorescently labeled secondary antibodies (Invitrogen) for 1 h at room temperature. Samples were counterstained with DAPI (Invitrogen) and imaged with an Olympus CKX41 microscope outfitted with a Leica DFC 3200 camera.

Londono R., Wenzhong W., Wang B., Tuan R.S, & Lozito T.P. (2017). Cartilage and Muscle Cell Fate and Origins during Lizard Tail Regeneration. Frontiers in Bioengineering and Biotechnology, 5, 70.

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Lizard Tail Regeneration Kinetics

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Lizards tail samples were collected after 0, 3, 7, 14, or 21 days following original tail amputation. Samples were fixed overnight in 4% paraformaldehyde, decalcified for 1 week in 10% EDTA (pH 7.4), equilibrated to 30% sucrose, embedded in optimal cutting temperature compound (OCT, Tissue-Tek), sectioned at 7 or 20 μm thickness, mounted on glass slides, and stained with 4′,6-diamidino-2-phenylindole (DAPI). For labeling of proliferating cell populations with 5-ethynyl-2’-deoxyuridine (EdU) (ThermoFisher Scientific), animals received IP injections of EdU (50 mg/kg) four hours prior to sample collection. Samples were cryosectioned and stained according to the manufacturer’s instructions. Images were captured with an Olympus CKX41 microscope outfitted with a Leica DFC 3200 camera. To measure phagocyte levels in vivo, histology samples were collected from lizards treated with DiI liposomes and imaged with a Keyence BZ-X800 microscope as 16 μm-thick Z-stacks, and the areas of DiI signal were quantified.

Londono R., Tighe S., Milnes B., DeMoya C., Quijano L.M., Hudnall M.L., Nguyen J., Tran E., Badylak S, & Lozito T.P. (2020). Single Cell Sequencing Analysis of Lizard Phagocytic Cell Populations and Their Role in Tail Regeneration.. Journal of immunology and regenerative medicine, 8, 100029.

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Histological Analysis of Calcium Deposition

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Calcium deposition in the implants was further examined histologically by Alizarin Red S staining (Rowley Biochemical, Danvers, MA) and then imaged with a CKX41 microscope (Olympus, Japan) equipped with a Leica DFC 3200 camera.

Lin H., Tang Y., Lozito T.P., Oyster N., Kang R.B., Fritch M.R., Wang B, & Tuan R.S. (2017). Projection Stereolithographic Fabrication of BMP-2 Gene-activated Matrix for Bone Tissue Engineering. Scientific Reports, 7, 11327.

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Histological Analysis of Muscle Tissue

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Muscle tissues were fixed in methanol and embedded in paraffin. For histology, sections (9 μm thick) were collected on Colorfrost Plus Microscope Slides (ThermoFisher Scientific) and rehydrated, and hematoxylin and eosin (H&E) staining was performed according to a routine Harris Hematoxylin and Eosin protocol. Immunohistochemistry (IHC) was performed according to protocols described for the Vectastain Elite ABC Kit (Vector Laboratories Inc., Burlingame, CA, USA). Images were taken using a CKX41 microscope (Olympus, Tokyo, Japan) equipped with a DFC 3200 camera (Leica, Wetzlar, Germany). For immunofluorescence staining, tissue sections were blocked in 10% bovine serum albumin for 20 min and then incubated overnight at 4 °C with mixed primary antibodies against BMP-7 (ab56023; Abcam) and CD68 (ab53444; Abcam). On the second day, sections were incubated with mixed secondary antibodies against rabbit IgG (ab150077; Abcam) and rat IgG (ab150158; Abcam) at room temperature for 1 h. After washing, the slides were mounted with 4′,6-diamidino-2-phenylindole (DAPI)-containing mounting medium (Vector Laboratories). Slides were viewed using an inverted IX81 microscope (Olympus) equipped with a Retiga EXi cooled CCD camera (Qimaging, Surrey, bc, Canada) and MetaMorph software (Molecular Devices, San Jose, CA, USA).

Li L., Jiang Y., Lin H., Shen H., Sohn J., Alexander P.G, & Tuan R.S. (2019). Muscle injury promotes heterotopic ossification by stimulating local bone morphogenetic protein-7 production. Journal of Orthopaedic Translation, 18, 142-153.

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Histochemical Analysis of Calcification

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Hematoxylin and Eosin (H&E) staining was performed according to routine Harris H&E protocol. For alizarin red staining, slides were stained with alizarin red solution (Alfa Aesar, Haverhill, MA, USA) for 45 s. The extent of calcification was evaluated by the ratio (%) of the calcium staining area to the total area, which could be calculated with the Image J version 1.5.0 software (National Institutes of Health). There were totally four region of interest every group and blinding was not performed. For Immunofluorescence (IF) staining, sections were blocked in 10% bovine serum albumin for 30 min and then incubated overnight at 4°C with mixed primary antibodies against ALP (ab83250, Abcam), OSX (ab22552, Abcam) and BMP-7 (ab56023, Abcam). Sections were incubated with fluorescein isothiocyanate-conjugated secondary antibody (ab150077, Abcam) for 1 h, then mounted with 4′, 6-diamidino-2-phenylindole (DAPI) on second day. The images were taken by an Olympus CKX41 microscope with a Leica DFC 3200 camera.

Cao G., L.i., Xiang S., Lin H., Pei F., Tuan R.S.C, & Alexander P.G. (2023). The development of a mouse model to investigate the formation of heterotopic ossification. Journal of orthopaedic surgery (Hong Kong), 31(1).

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