Na 1.45 oil immersion phase contrast objective

Manufactured by Nikon

Sourced in Japan

The 100X (NA = 1.45) oil immersion phase contrast objective is a specialized optical component designed for microscopy applications. It provides a magnification of 100X and a numerical aperture (NA) of 1.45, which allows for high-resolution imaging. The phase contrast feature enhances the visibility of transparent or low-contrast samples. This objective is intended for use with oil immersion techniques to further improve image quality and resolution.

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Lab products found in correlation

Tie inverted epifluorescence microscope, by Nikon (2 mentions) Nis elements software, by Nikon (2 mentions) Ixon du 897 emccd camera, by Oxford Instruments (2 mentions)

Spelling variants of this product

60× oil immersion objective (38 citations) 100x/NA 1.45 oil objective (7 citations) 100×/1.49 NA oil-immersion objective (5 citations) 100x NA 1.45 objective (4 citations) 100× 1.45 NA oil immersion objective (2 citations)

2 protocols using na 1.45 oil immersion phase contrast objective

Bacteria Imaging with Fluorescence Microscopy

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A Nikon Ti-E inverted epifluorescence microscope (Nikon Instruments, Japan) with a 100X (NA = 1.45) oil immersion phase contrast objective (Nikon Instruments, Japan) is used for imaging the bacteria. Images are captured on an iXon DU-897 EMCCD camera (Andor Technology, UK) and recorded using NIS-Elements software (Nikon Instruments, Japan). Fluorophores are excited by a 200 W Hg lamp through an ND4 neutral density filter. A Chroma 41004 filtercube (Chroma Technology Corp., VT) is used when capturing fluorescent images. A motorized stage and a perfect focus system are utilized during time-lapse imaging. About 100 dead-end channels for each channel size is imaged in each measurement for 6-h period or longer (at least 5 doublings).

Yang D., Jennings A.D., Borrego E., Retterer S.T, & Männik J. (2018). Analysis of Factors Limiting Bacterial Growth in PDMS Mother Machine Devices. Frontiers in Microbiology, 9, 871.

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Multicolor Fluorescence Microscopy Setup

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A Nikon Ti-E inverted epifluorescence microscope (Nikon Instruments, Japan) with a 100X (NA = 1.45) oil immersion phase contrast objective (Nikon Instruments, Japan), was used for imaging the bacteria. Images were captured on an iXon DU897 EMCCD camera (Andor Technology, Ireland) and recorded using NIS-Elements software (Nikon Instruments, Japan). Fluorophores were excited by a 200W Hg lamp through an ND8 neutral density filter. A Chroma 41004 filtercube was used for capturing mCherry and tag-RFP-T images, and a Chroma 41001 (Chroma Technology Corp., VT) for mNeonGreen images. A motorized stage and a perfect focus system were utilized throughout time-lapse imaging.

Yang D., Männik J., Retterer S.T, & Männik J. (2020). The effects of polydisperse crowders on the compaction of the Escherichia coli nucleoid. Molecular microbiology, 113(5), 1022-1037.

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