Palmitic acid

Manufactured by PerkinElmer

Sourced in United States

Palmitic acid is a long-chain saturated fatty acid. It is a common component of animal and vegetable fats and oils.

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Lab products found in correlation

14c palmitic acid, by PerkinElmer (1 mentions) Palmitic acid, by Merck Group (1 mentions) 9 10 3h n palmitic acid, by PerkinElmer (1 mentions) Bsa conjugated palmitic acid, by Merck Group (1 mentions)

Close spelling variants of this product

14C-palmitic acid [14C(U)]-palmitic acid [1-14C] palmitic acid [3H]-palmitic acid [9,10-3H]-palmitic acid [9,10-3H(N)]-palmitic acid

3 protocols using palmitic acid

Lipid Metabolism Assays in Cells

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For lipid accumulation, cells were pre-incubated overnight
(~16 h) in DMEM/F12 + 2.5% FBS (no supplements) followed by
incubatition with 500 μM palmitic acid (Sigma, catalog P5585)
pre-complexed with fatty acid-free bovine serum albumin (FAF-BSA) or
BSA-only as controls for 8 h. Total intracellular triglycerides were
measured by an enzymatic plate-based assay (Pointe Scientific) and
normalized to protein content. For FAO, cells were serum-starved in the
presence of 0.1% FAF-BSA for 3 h, followed by 1 h with fatty acid incubation
media (FAIM) containing 20 μM palmitic acid conjugated with FAF-BSA
plus 1 h in the presence of 0.1 μCi ¹⁴C palmitic acid
(Perkin Elmer). FAO was determined as the fraction of ¹⁴C
palmitic acid-derived CO₂ trapped in filter papers normalized to
protein content as previously described (Akie
and Cooper, 2015). Before addition of ¹⁴C palmitic
acid, an aliquot of FAIM was collected and assayed for
β-hydroxybutyrate levels using a fluorimetric method (Cayman
Chemical) according to manufacturer’s instructions. For fatty acid
uptake, cells were serum-starved for 3 h and incubated with FAIM containing
200 μM palmitic acid conjugated with FAF-BSA for 30 min plus another
30 min in the presence of 0.1 μCi ¹⁴C palmitic acid. Fatty
acid uptake was interpreted as protein-normalized counts from cell
lysates.

Batista T.M., Garcia-Martin R., Cai W., Konishi M., O’Neill B.T., Sakaguchi M., Kim J.H., Jung D.Y., Kim J.K, & Kahn C.R. (2019). Multi-dimensional Transcriptional Remodeling by Physiological Insulin In vivo. Cell reports, 26(12), 3429-3443.e3.

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Intestinal Fatty Acid Oxidation Assay

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Fatty acid oxidation activity was measured as we have previously described [25] (link). After 6 weeks of HFD feeding, mice were fasted for 4 h. The second segment of freshly collected small intestine (200 mg) was homogenized in 600 µl of 0.25 M sucrose containing 1 mM EDTA. The tissue homogenate (1 mg protein) was incubated for 30 min at 25°C in a buffer containing 150 mM KCl, 10 mM HEPES (pH 7.2), 0.1 mM EDTA, 1 mM potassium phosphate buffer (pH 7.2), 5 mM malonate, 10 mM MgCl₂, 1 mM carnitine, 0.15% fatty acid free-BSA, 5 mM ATP, and 50 µM palmitic acid containing 1 µCi of [9, 10-³H(N)] palmitic acid (32.4 Ci/mmol; PerkinElmer, Boston, MA). Reactions were stopped by addition of 200 µL 0.6 N perchloric acid. After removal of unreacted fatty acids by hexane extraction, acid-soluble radiolabeled degradation products in the aqueous phase were measured by liquid scintillation counting and the rates of fatty acid oxidation were presented as pmol/min/mg tissue protein.

Xie P., Guo F., Ma Y., Zhu H., Wang F., Xue B., Shi H., Yang J, & Yu L. (2014). Intestinal Cgi-58 Deficiency Reduces Postprandial Lipid Absorption. PLoS ONE, 9(3), e91652.

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Palmitic Acid Oxidation in Hepatocytes

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Hepatocytes were incubated in DMEM supplemented with 1 g/L glucose, 12.5 μM BSA-conjugated palmitic acid (Sigma Aldrich), and 2.5 μCi palmitic acid [9,10-3H(N)] (Perkin Elmer, USA). Four hours later, 0.2 mL of media were collected to 2 mL tubes containing 0.8 mL of activated charcoal slurry. Samples incubated at room temperature for 30 min with frequent mixing. After centrifugation at 13,000 rpm for 15 min, 0.2 mL of supernatant were transferred to 4 mL scintillation vials containing 2.8 mL of Ultima FLO M scintillation liquid (Perkin Elmer, USA) and measured in a Wallac Winspectral 1414 liquid scintillation counter. Cells were lysed in 0.03% SDS and protein concentration was determined for each lysate. The FA oxidation rate was normalized by cellular protein content.

Correia J.C., Massart J., de Boer J.F., Porsmyr-Palmertz M., Martínez-Redondo V., Agudelo L.Z., Sinha I., Meierhofer D., Ribeiro V., Björnholm M., Sauer S., Dahlman-Wright K., Zierath J.R., Groen A.K, & Ruas J.L. (2015). Bioenergetic cues shift FXR splicing towards FXRα2 to modulate hepatic lipolysis and fatty acid metabolism. Molecular Metabolism, 4(12), 891-902.

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