Log In Sign up
The largest database of trusted experimental protocols

SOCS1 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is a protein that functions as a negative regulator of cytokine signaling pathways.

Automatically generated - may contain errors

Lab products found in correlation

M mlv reverse transcriptase, by Thermo Fisher Scientific (2 mentions) Ripa lysis buffer, by Thermo Fisher Scientific (2 mentions) Ifi16, by Thermo Fisher Scientific (1 mentions) Fx96 cycler, by Bio-Rad (1 mentions) Ifi44, by Thermo Fisher Scientific (1 mentions) Ifih1, by Thermo Fisher Scientific (1 mentions) Taqman primer probe set, by Thermo Fisher Scientific (1 mentions) Mgcl2, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Tapestation 4200, by Agilent Technologies (1 mentions) Ifit1, by Cell Signaling Technology (1 mentions) Usp18, by Cell Signaling Technology (1 mentions) Stat2, by Merck Group (1 mentions) Phospho tyr 701 stat1, by Cell Signaling Technology (1 mentions) Stat1, by Santa Cruz Biotechnology (1 mentions) Protease phosphatase inhibitor cocktail, by Cell Signaling Technology (1 mentions) Gapdh, by Merck Group (1 mentions) Micro bca protein assay, by Thermo Fisher Scientific (1 mentions) β actin, by ABclonal (1 mentions)

2 protocols using socs1

1

Quantifying Immune Response Transcripts in Neutrophils

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated using the RNeasy Mini kit following the manufacturer’s instructions (Qiagen, Hilden, Germany). RNA quality and quantification were determined by TapeStation 4200 (Agilent, St. Clara, USA) following the manufacturer’s instructions. Neutrophil total RNA was isolated using RNeasy Mini kit following the manufacturer’s instructions (Qiagen), and complementary DNA (cDNA) was synthesized using M-MLV Reverse Transcriptase (Thermo Fisher Scientific). RT-PCR was performed in duplicates using the cDNA and Platinum Taq polymerase (Thermo Fisher Scientific), 200 nM dNTP (Promega, Southampton, UK), 50mM MgCl2 (Thermo Fisher Scientific), and TaqMan primer/probe sets (Thermo Fisher Scientific). Samples were matched to a standard curve generated by amplifying serially diluted products using the same PCR reaction and normalized to GAPDH (Hs00266705_g1) to obtain the relative expression value. Real-time assays were run on FX96 Cycler (Bio-Rad). The following are the primes used: STAT1 (Hs01013996_m1), STAT2 (Hs01013115_g1), STAT3 (Hs00374280_m1), IFIH1 (Hs00223420_m1), IFIT (Hs00356631_g1), ISG15 (Hs00192713_m1), MX1 (Hs00895608_m1), IRF1 (Hs00971965_m1), SOCS1 (Hs00705164_s1), USP18 (Hs00276441_m1), IFI44 (Hs00197427_m1), IFI16 (Hs00986757_m1), and OAS (Hs00242943_m1) (all from Thermo Fisher Scientific).
Parackova Z., Vrabcova P., Zentsova I., Sediva A, & Bloomfield M. (2023). Neutrophils in STAT1 Gain-Of-Function Have a Pro-inflammatory Signature Which Is Not Rescued by JAK Inhibition. Journal of Clinical Immunology, 43(7), 1640-1659.
+ Open protocol
+ Expand
2

Immunoblotting of JAK-STAT Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-cell extracts for immunoblotting were prepared by incubating cells for 20 min in RIPA lysis buffer (Thermo Fisher Scientific) with 50 mM dithiothreitol and Protease/Phosphatase inhibitor cocktail (Cell Signaling Technology). Protein was quantified with Micro BCA Protein Assay (Thermo Fisher Scientific). Immunoblotting was performed using the Bio-Rad Western blot workflow. Membranes were blocked in 5% BSA for primary antibodies or 5% nonfat dry milk for secondary antibodies. Antibodies used were STAT1 (Santa Cruz Biotechnology), STAT2 (Millipore), phospho-Tyr 701 STAT1 (Cell Signaling Technology), phospho-Tyr 689 STAT2 (Cell Signaling Technology), USP18 (Cell Signaling Technology), IFIT1 (Cell Signaling Technology), MX1 (Abcam), SOCS1 (Thermo Fisher Scientific), β-actin (ABclonal), and GAPDH (Millipore). Signal was detected with enhanced chemiluminescence detection reagent (ECL or SuperSignal West pico, Thermo Fisher Scientific) by film development or capturing on ImageQuant 800 Fluor imaging system (Cytiva). For all representative immunoblots shown, n=3 separate experiments were carried out.
Malle L., Martin-Fernandez M., Buta S., Richardson A., Bush D, & Bogunovic D. (2022). Excessive Negative Regulation of Type I Interferon Disrupts Viral Control in Individuals with Down Syndrome. Immunity, 55(11), 2074-2084.e5.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!