Lasb2 link biotin and streptavidin hrp

Sections were stained as previously described [5 (link)]. Briefly, paraffin-embedded tissue sections were deparaffinized and then either directly stained with hematoxylin and eosin (H&E) or immersed in retrieval solution (Dako) for 20 minutes at 90–99°C. Slides were incubated at room temperature for 30 minutes with anti-human CD31 antibody (clone JC70/A, Abcam) followed by a 10-minute incubation with LASB2 link-biotin and streptavidin-HRP (Vector Laboratories), then developed with DAB (Vector Laboratories) solution for 5 minutes. Slides were analyzed by microscope under 20× magnification.

Sections were stained as previously described [27 (link)]. Briefly, paraffin-embedded tissue sections were deparaffinized and then either directly stained with hematoxylin and eosin (H&E) or immersed in retrieval solution (Dako) for 20 min at 90–99°C. Slides were incubated at room temperature for 30 min with anti-human CD31 antibody (clone JC70/A, Abcam), and rat anti-mouse smooth muscle α actin (αSMA) (clone 1A4, Sigma) followed by a 10-minute incubation with LASB2 link-biotin and streptavidin-HRP (Vector Laboratories), then developed with DAB (Vector Laboratories) solution for 5 min. Slides were analyzed by microscope under 40× magnification.