Anti nfatc1 antibody 7a6

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Anti-NFATc1 antibody (7A6) is a laboratory research tool. It is designed to detect the presence and localization of the transcription factor NFATc1 in biological samples.

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Lab products found in correlation

Benzonase nuclease, by Merck Group (1 mentions) Anti gapdh antibody, by Merck Group (1 mentions) Ripa lysis and extraction buffer, by Thermo Fisher Scientific (1 mentions) Ecl western blot substrate, by Merck Group (1 mentions) Trans blot turbo system, by Bio-Rad (1 mentions) Las 3000 mini, by Fujifilm (1 mentions) Fitc anti human cd14, by BioLegend (1 mentions) Tumor necrosis factor (tnf), by R&D Systems (1 mentions) Isotype mouse igg1 fitc, by BioLegend (1 mentions) Soluble rankl, by R&D Systems (1 mentions) Il 23, by R&D Systems (1 mentions)

Spelling variants of this product

NFATc1 (11 citations) Anti-NFATc1 (4 citations)

2 protocols using anti nfatc1 antibody 7a6

Western Blot Analysis of NFATc1 Expression

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For protein extraction, RIPA Lysis and Extraction buffer (Thermo Fisher Scientific), supplemented with Halt Protease and Phosphatase Inhibitor Cocktail, phenylmethylsulfonylfluoride (PMSF) and Benzonase Nuclease (Merck, Darmstadt, Germany) was used according to the manufacturer’s protocol. Fifteen micrograms protein were resolved by gel electrophoresis and blotted to nitrocellulose using a Trans-Blot Turbo System (Bio-Rad, Feldkirchen, Germany). Membranes were blocked with 5% non-fat milk and incubated with anti-NFATc1 antibody (7A6; Thermo Fisher Scientific) or anti-GAPDH antibody (Sigma-Aldrich) overnight at 4 °C. After incubation with the corresponding secondary antibody (anti-mouse or anti-rabbit IgG (H+L) peroxidase; Vector Laboratories, Burlingame, CA, USA), blots were developed by means of ECL Western blot substrate (Merck). Images were acquired with a luminescence imager LAS 3000 mini (FujiFilm, Düsseldorf, Germany).

Wu Y., Kröller L., Miao B., Boekhoff H., Bauer A.S., Büchler M.W., Hackert T., Giese N.A., Taipale J, & Hoheisel J.D. (2021). Promoter Hypermethylation Promotes the Binding of Transcription Factor NFATc1, Triggering Oncogenic Gene Activation in Pancreatic Cancer. Cancers, 13(18), 4569.

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Osteoclastogenesis Signaling Pathway Assay

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Soluble RANKL, OPG, IL-23, TNF, RANKL ELISA and anti-MDL-1/CLEC5A antibody
(283834) were purchased from R&D Systems (USA). Anti-phospho-SYK (Y525/526, Y323,
Y352), anti-PLCγ2 (polyclonal), and anti-phospho-PLCγ2 (Y759) antibodies
were purchased from Cell Signaling (USA). Anti-SYK antibody (SYK-01) was purchased from
Abcam (UK). Anti-GAPDH, (6C5) anti-SRC (GD11), anti-phospho-SRC (Y418),
anti-phosphotyrosine (4G10) antibodies were purchased from Millipore (USA). Anti-human
CD16 PE-cy7 (clone CB16), isotype Mouse IgG1κ and anti-human CD16 PE-cy5 (clone
3G8) and isotype mouse IgG1 PE-cy5 were from Ebioscience USA and Biolegend USA
respectively. Anti-human CD14 FITC and isotype Mouse IgG1 FITC were from Biolegend, USA.
Anti-human DAP-12 PE and isotype control Rat IgG1 PE were purchased from Beckman Coulter
and Ebioscience, USA. Anti-NFATc1 antibody (7A6) was purchased from Thermo Scientific
(USA). Anti-DAP12 antibody (406288) was purchased from Exalpaha (USA). Anti-IL-23R (H-300)
was purchased from Santa Cruz (USA).

SHIN H.S., SARIN R., DIXIT N., WU J., GERSHWIN M.E., BOWMAN E.P, & ADAMOPOULOS I.E. (2014). Crosstalk among interleukin 23 and DNAX activating protein 12-dependent pathways promotes osteoclastogenesis. Journal of immunology (Baltimore, Md. : 1950), 194(1), 316-324.

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