α amylase α ams assay kit

High-glucose Dulbecco's modified Eagle's medium was obtained from HyClone Co (Logan, Utah). Fetal bovine serum, trypsin-Ethylene Diamine Tetraacetic Acid (0.05%), and phenol red were provided by Gibco Co, Ltd (Thermo Fisher Scientific, Cleveland, Ohio). Emodin and STC were purchased from Solarbio Science and Technology Co (Beijing, China). PrimeScript RT reagent and SYBR PremixEx Taq II (TliRNaseH Plus) were purchased from TaKaRa Biotechnology Co, Ltd (Dalian, China). RNAex Pro RNA reagent was obtained from Accurate Biology Co, Ltd (Changsha, China). A ROS Detection Assay Kit was purchased from AmyJet Scientific Inc Co, Ltd (Wuhan, China). An α-Amylase (α-AMS) Assay Kit and Gamma-Glutamylcysteine Synthetase (γ-GCS) Assay Kit were provided by the Nanjing Jiancheng Institute of Biotechnology (Nanjing, China). A Micro Reduced Glutathione (GSH) Assay Kit was provided by Solarbio Science and Technology Co (Beijing, China). Rabbit anti-S100A9 was purchased from Cell Signaling Technology, Inc (Beverly, Mass). Rabbit anti-VNN1 was purchased from the Proteintech Group (Chicago, Ill). Goat antirabbit IgG H&L (Horseradish Peroxidase) was obtained from Abcam (Cambridge, UK). An Annexin V-FITC/PI Apoptosis Detection Kit was provided by Dalian Meilun Biotechnology Co, Ltd (Dalian, China).