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3 protocols using 3 h tiotidine

1

Radioligand binding assay protocols

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Cell culture medium, antibiotics, isobutylmethyl xanthine (IBMX), cAMP, bovine serum albumin (BSA), cycloheximide, amthamine, famotidine, forskolin, and PD98059 were obtained from Sigma Chemical Company (St. Louis, MO, USA). Tiotidine were from Tocris Cookson Inc. (Ballwin, MO, USA). [3H]cAMP, and [3H]tiotidine were purchased from Perkin Elmer Life Sciences (Boston, MA, USA). Fetal calf serum was from Natocor (Argentina). Other chemicals used were of analytical grade and obtained from standard sources.
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2

Immune Cell Signaling Modulation

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Cell culture medium, antibiotics, isobutylmethyl xanthine (IBMX), cAMP, prostaglandin E2 (PGE2), 2,3-trifluormetilfenilhistamine dimaleate (histamine H 1 receptor agonist), bovine serum albumin (BSA), phorbol 12-myristate 13-acetate (PMA), lipopolysaccharides from Escherichia Coli (LPS), cimetidine, ranitidine hydrochloride, famotidine, cetirizine dihydrocloride, chlorpheniramine maleate and diphenhydramine hydrochloride were obtained from Sigma Chemical Company (St.
Louis, MO). Amthamine dihydrobromide, mepyramine maleate, triprolidine hydrochloride, tiotidine and dynasore were acquired from Tocris Cookson Inc.
(Ballwin, MO). [ 3 H]cAMP, [ 3 H]mepyramine and [ 3 H]tiotidine, were purchased from Perkin Elmer Life Sciences (Boston, MA). Fetal bovine serum (FBS) was purchased from Natocor (Argentina). Other chemicals used were of analytical grade and obtained from standard sources.
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3

Radioligand Binding Assay for Histamine Receptors

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DMEM medium, antibiotics, phosphate-buffered saline (PBS), amthamine and bovine serum albumin (BSA) were obtained from Sigma. Tiotidine, cimetidine, famotidine and ranitidine were from Tocris Bioscience (UK). Fetal bovine serum (FBS) was purchased from Natocor (Córdoba, Argentina). All other chemicals were of analytical grade and obtained from standard sources. [ 3 H]cAMP and [ 3 H]Tiotidine were purchased from PerkinElmer Life Sciences (Boston, MA, USA).
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