Solvents were used as received. Solution
1H,
1H–
1H COSY, NOESY NMR spectra were recorded using a Bruker
Avance 400 and
AMX-400 spectrometers. Mass spectra were measured on a Bruker
maXis II ESI-QTOF instrument in the ESI
+ mode. Microanalyses were carried out in the analytical laboratory of the University of Eastern Finland. The bis(μ-chlorido) bridged dimeric precursor {(N^C–COOMe)
2IrCl}
2 (N^C–COOMe = methyl 2-phenylquinoline-4-carboxylate) were synthesized according to published procedures.
32 (link) Methyl 2-phenylquinoline-4-carboxylate, 1,2-dipalmitoyl-
sn-glycero-3-phosphocholine (DPPC) and 1,2-distearoyl-
sn-glycero-3-phosphoethanolamine-
N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000) were purchased from Sigma-Aldrich (St. Louis, MO, USA). HEPES buffer solution contained 20 mM HEPES and 140 mM NaCl in MilliQ purified water and the pH was adjusted to 7.4 with NaOH. Calcein solution had 60 mM of calcein and 29 mM of NaCl in MilliQ purified water and the pH was set to 7.4 with NaOH.
Shakirova J.R., Sadeghi A., Koblova A.A., Chelushkin P.S., Toropainen E., Tavakoli S., Kontturi L.S., Lajunen T., Tunik S.P, & Urtti A. (2020). Design and synthesis of lipid-mimetic cationic iridium complexes and their liposomal formulation for in vitro and in vivo application in luminescent bioimaging. RSC Advances, 10(24), 14431-14440.