The uninfected, LV-NC and LV-miR-137 groups of HTR-8/SVneo cells were plated at a density of 600-800 cells/well into a 6-well culture plate (Corning Incorporated) and cultured at 37°C in an atmosphere containing 5% CO2, with exchange of the culture medium every other day. The uniformly plated uninfected HTR-8/SVneo cells were cultured with IL-6 antibody (α-IL-6; 1:1,000; BioLegend, Inc.) supplementary medium at 37°C, with HTR-8/SVneo cells not treated with α-IL-6 as a control group. The LV-NC and LV-miR-137 HTR-8/SVneo cells were cultured with normal and HG medium. After 10 days, the medium was removed from each well, and the plate was washed with PBS. The cells were then fixed with 4% paraformaldehyde (Biotech Well, Shanghai, China) for 10 min. Following the fixation process, crystal violet hydrate solution (Sangong Biotech, Co., Ltd.) was added for cell staining for 30 min. Finally, images of each well were captured by a camera (E-M1 Mark II; Olympus Corporation;) and colonies were counted, with a colony defined as >15 cells.
E m1 mark 2
Manufactured by Olympus
The E-M1 Mark II is a digital camera produced by Olympus. It features a 20.4 megapixel Micro Four Thirds sensor, 5-axis in-body image stabilization, and a 121-point autofocus system. The camera is capable of capturing high-quality images and video.
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Lab products found in correlation
3 protocols using e m1 mark 2
1
Evaluating miR-137 Effects on HTR-8/SVneo Cells
2
Morphological Comparison of Parasitic Wasps
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NMLU = Natur-Museum, Lucerne, Switzerland
PRC = Paolo Rosa Collection, Bernareggio, Italy
TWC = Thomas J. Wood Collection, Mons, Belgium
ICC = Ian Cross Collection, Briantspuddle, Dorset, United Kingdom
In the text, the following abbreviations are used for morphological terms:
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In the text, the following abbreviations are used for morphological terms:
F1, F2, F3 = flagellomeres 1, 2, 3
MOD = anterior ocellar diameter
MS = malar space, the shortest distance between base of mandible and margin of compound eye
OOL = oculo-ocellar line, the shortest distance between lateral ocellus and compound eye
P = pedicel
PD = puncture diameter
POL = the shortest distance between posterior ocelli
T1–T3 = metasomal terga 1 to 3
vs. = versus
3
Bee Morphology and Dietary Analysis
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Morphological terminology follows MICHENER (2007) . The abbreviations A and T are used for antennal segments and metasomal terga, respectively. Specimens were measured from the vertical plane of the front of the head to the tip of the metasoma. Photographs were taken using an Olympus E-M1 Mark II with a 60 mm macro lens. Close-ups were taken with the addition of a Mitutoyo M Plan Apo 10X infinity corrected objective lens or a Lomo 3.7x objective lens in combination with an Olympus M.Zuiko 2x teleconverter lens, a 10 mm Kenko DG extension tube, and a Meike MK-P-AF3B 10 mm extension tube. Photographs were stacked using Zerene Stacker ver. 1.04 (Zerene Systems, USA) and plates were prepared in GNU Image Manipulation Program (GIMP) ver. 2.10. Post-processing of some images was made in Photoshop Elements (Adobe Systems, USA) in order to improve lighting to highlight specific characters.
Where available, pollen was removed from scopae of female Andrena and analysed by light microscopy in order to investigate their dietary preferences following the method of WOOD & ROBERTS (2018) (link). Dietary classification follows MÜLLER & KUHLMANN (2008) (link).
Where available, pollen was removed from scopae of female Andrena and analysed by light microscopy in order to investigate their dietary preferences following the method of WOOD & ROBERTS (2018) (link). Dietary classification follows MÜLLER & KUHLMANN (2008) (link).
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