Cobas taqman hiv 1 assay

Medical records were carefully reviewed, and all subjects underwent a physical examination. Information on gender, age, body mass index (BMI), smoking status, family history of cardiovascular disease, and treatment with antiretroviral drugs was recorded. The presence of arterial hypertension, hypercholesterolemia, and hypertriglyceridemia was defined according to the Adult Treatment Panel III criteria [15 (link)]. Dyslipidemia was considered if total cholesterol was ≥200 mg/dL, LDL cholesterol was ≥130 mg/dL, or HDL cholesterol was <40 mg/dL. A sample of fasting venous blood was obtained to determine concentrations of glucose, high-sensitivity C-reactive protein (hsCRP), creatinine, total cholesterol, D-dimer, high-density lipoprotein (HDL) cholesterol, and triglycerides using standard enzymatic methods. Low-density lipoprotein (LDL) cholesterol concentrations were calculated using the Friedewald equation. Plasma viral load was measured using the Cobas TaqMan HIV-1 assay (Roche Diagnostics Systems, Branchburg, NJ, USA). CD4 and CD8 T-cell counts were determined by flow cytometry (Becton Dickinson [BD], San Jose, CA, USA).