Log In Sign up
The largest database of trusted experimental protocols

Platinum sybr green ppcr supermix udg with rox

Manufactured by Thermo Fisher Scientific
Visit Supplier

Platinum SYBR green pPCR SuperMIX-UDG with ROX is a ready-to-use solution for qPCR analysis. It contains all the necessary components, including SYBR Green I dye, Uracil-DNA Glycosylase (UDG), and ROX passive reference dye, to perform real-time PCR amplification and detection.

Automatically generated - may contain errors

Lab products found in correlation

Taqman universal master mix 2 with ung, by Thermo Fisher Scientific (2 mentions) 7a900ht fast real time pcr system, by Thermo Fisher Scientific (2 mentions) Superscript 3 first strand synthesis supermix, by Thermo Fisher Scientific (2 mentions) Rneasy mini kit, by Qiagen (2 mentions) Random primer, by Thermo Fisher Scientific (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Superscript 3 reverse transcriptase, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Platinum SYBR Green SuperMix-UDG SYBR Green Supermix with ROX SYBR Green Platinum SuperMix Platinum SYBR Green SYBR Green Supermix SYBR Green

3 protocols using platinum sybr green ppcr supermix udg with rox

1

Quantitative RT-PCR of cIAP2 Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using TRIzol (Invitrogen, Carlsbad, CA, USA) and reverse transcription using random primers (Invitrogen) was performed on 2μg total RNA using SuperScript™ III reverse transcriptase (Invitrogen) both according to manufacturer's instructions. cDNA was analyzed by quantitative real-time RT-PCR using Platinum® SYBR®Green pPCR Supermix UDG with Rox (Invitrogen) and 0.3mM of cIAP2 forward primer 5′-gtg tta gac tta ctc aat gca gaa g-3′ and 0.3mM of reverse primer 5′-cca gga ttg gaa tta cac aag tc-3′. The run and analysis were performed using Mx3005P (Stratagene).
Duvefelt C.F., Lub S., Agarwal P., Arngården L., Hammarberg A., Maes K., Van Valckenborgh E., Vanderkerken K, & Wiklund H.J. (2015). Increased resistance to proteasome inhibitors in multiple myeloma mediated by cIAP2 - implications for a combinatorial treatment. Oncotarget, 6(24), 20621-20635.
+ Open protocol
+ Expand
2

RNA Extraction and qRT-PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was isolated using the RNeasy Mini Kit (Qiagen) including on-column DNase digest to remove genomic DNA. Reverse transcription was performed on 0.5–1μg of total RNA using oligo dT priming and SuperScript® III First-Strand Synthesis SuperMix (Life technologies) at 50°C according to providers instructions. All PCR reactions were performed in a 384-well plate format on a 7A900HT Fast Real-Time PCR system (Applied Biosystems) using either Taqman® Universal Master MIX II with UNG (Applied Biosystems) or Platinum SYBR green pPCR SuperMIX-UDG with ROX (Invitrogen) according to manufacturers’ instructions using primer as summarized in Supplementary Table 3c. Relative quantification of gene expression was calculated using the 2–[delta][delta]Ct method using GAPDH (Taqman®) or 60S acidic ribosomal protein P0 (RPLP0 for SYBR green) as reference and untreated control samples (as specified in the Figure legends) as calibrator.
Soldner F., Stelzer Y., Shivalila C.S., Abraham B.J., Latourelle J.C., Barrasa M.I., Goldmann J., Myers R.H., Young R.A, & Jaenisch R. (2016). Parkinson-associated risk variant in enhancer element produces subtle effect on target gene expression. Nature, 533(7601), 95-99.
+ Open protocol
+ Expand
3

RNA Extraction and qRT-PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was isolated using the RNeasy Mini Kit (Qiagen) including on-column DNase digest to remove genomic DNA. Reverse transcription was performed on 0.5–1μg of total RNA using oligo dT priming and SuperScript® III First-Strand Synthesis SuperMix (Life technologies) at 50°C according to providers instructions. All PCR reactions were performed in a 384-well plate format on a 7A900HT Fast Real-Time PCR system (Applied Biosystems) using either Taqman® Universal Master MIX II with UNG (Applied Biosystems) or Platinum SYBR green pPCR SuperMIX-UDG with ROX (Invitrogen) according to manufacturers’ instructions using primer as summarized in Supplementary Table 3c. Relative quantification of gene expression was calculated using the 2–[delta][delta]Ct method using GAPDH (Taqman®) or 60S acidic ribosomal protein P0 (RPLP0 for SYBR green) as reference and untreated control samples (as specified in the Figure legends) as calibrator.
Soldner F., Stelzer Y., Shivalila C.S., Abraham B.J., Latourelle J.C., Barrasa M.I., Goldmann J., Myers R.H., Young R.A, & Jaenisch R. (2016). Parkinson-associated risk variant in enhancer element produces subtle effect on target gene expression. Nature, 533(7601), 95-99.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!