L glutamine

Manufactured by Vetec

Sourced in Brazil

L-glutamine is a non-essential amino acid that plays a vital role in various biological processes. It is a key component in the synthesis of proteins and serves as a precursor for other important molecules, such as glutathione and glutamic acid. L-glutamine is involved in maintaining the integrity of the gastrointestinal tract and supporting immune system function.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Hepes, by Thermo Fisher Scientific (2 mentions) Sodium bicarbonate, by Vetec (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Amphotericin b, by Merck Group (1 mentions) Trypsin edta solution, by Thermo Fisher Scientific (1 mentions) Mycoplasma stain kit, by Merck Group (1 mentions) β mercaptoethanol, by Merck Group (1 mentions)

3 protocols using l glutamine

Maintaining Cell Lines for Multidrug Resistance

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Cells and culture procedures
The K562, Lucena-1 and FEPS cell lines were provided of Immunology Laboratory at the Medical Biochemistry Institute Leopoldo de Meis of the Federal University of Rio de Janeiro (Brazil). The cells were were cultured in the Cell Culture Laboratory of the Federal University of Rio Grande at 37 • C in 5% CO 2 in disposable plastic flasks containing RPMI 1640 (Gibco, Brazil), medium supplemented with sodium bicarbonate (2.0 g/L) (Vetec, Brazil), l-glutamine (0.3 g/L) (Vetec, Brazil), Hepes (25 mM) (Acros, Belgium), 10% fetal bovine serum (Gibco, Brazil), 1% antibiotic (penicillin, 100 U/mL and streptomycin 100 mg/mL) (Gibco, Brazil) and antimycotic (amphotericin B 0.25 mg/mL -Sigma, Brazil). For maneitance of MDR phenotype, Lucena-1 cell line was maintained with 60 nM of VCR (Sigma, Brazil) and FEPS cell line with 300 ng/ml of DNR (Sigma, Brazil).

Marques M.B., Andrade F.R.T., Silva E.F.E., Oliveira B.R., Almeida D.V., de Souza Votto A.P, & Marins L.F. (2023). Effects of chemotherapeutic drugs on the antioxidant capacity of human erythroleukemia cells with MDR phenotype. Molecular and cellular biochemistry, 478(11).

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Characterization of Cancer Cell Lines

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A total of eight cancer cell lines and two non-cancer cells were used in this study and the detailed are shown in Table S1. Primary cell culture of peripheral blood mononuclear cells (PBMC) were obtained with informed consent (# 031019/2013). Cells were cultured in RPMI-1640 medium (Gibco-BRL, Gaithersburg, MD, USA) with 10% fetal bovine serum (Life, Carlsbad, CA, USA), 2 mM L-glutamine (Vetec Química Fina, Duque de Caxias, RJ, Brazil) and 50 μg/mL gentamycin (Life, Carlsbad, CA, USA). Adherent cells were collected by treatment with 0.25% trypsin EDTA solution (Gibco-BRL, Gaithersburg, MD, USA). All cell lines were cultured in flasks at 37 °C in 5% CO₂ and sub-cultured every 3–4 days to maintain exponential growth. All cell lines were tested for mycoplasma using a mycoplasma stain kit (Sigma-Aldrich Co.) to validate the use of cells free from contamination. Cell viability was assessed by trypan blue exclusion assay for all experiments and over 90% of the cells were viable at the beginning of the culture.

Oliveira M.D., Barbosa M.I., de Souza T.B., Moreira D.R., Martins F.T., Villarreal W., Machado R.P., Doriguetto A.C., Soares M.B, & Bezerra D.P. (2018). A novel platinum complex containing a piplartine derivative exhibits enhanced cytotoxicity, causes oxidative stress and triggers apoptotic cell death by ERK/p38 pathway in human acute promyelocytic leukemia HL-60 cells. Redox Biology, 20, 182-194.

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Cytotoxicity of Microcystins in HTC Cells

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The HTC cell line was obtained from the Cell Bank of Rio de Janeiro, Brazil. HTC cells were grown in RPMI 1640 medium (Gibco, USA) supplemented with sodium bicarbonate (0.2 g/L) (Vetec, Brazil), L-glutamine (0.3 g/L) (Vetec, Brazil), Hepes (25 mM) (Acros, USA) and b-mercaptoethanol (5 × 10–5 M) (Sigma, Germany), with 10 % fetal bovine serum (Gibco, Brazil), 1 % of antibiotic and antimycotic (penicillin – 100 U/mL, streptomycin-100 mg/mL and amphotericin B - 0.25 mg/mL), in disposable plastic flasks, at 37°C.
The cytotoxicity assay was performed by trypan blue exclusion after 24 hours of incubation with microcystins. Three independent experiments were carried out using triplicates in each experiment. Data are expressed as mean + standard error and analyzed using ANOVA followed by Tukey’s multiple range test. Significance level was fixed in 0.05.

Ramos D.F., Matthiensen A., Colvara W., de Votto A.P., Trindade G.S., da Silva P.E, & Yunes J.S. (2015). Antimycobacterial and cytotoxicity activity of microcystins. The Journal of Venomous Animals and Toxins Including Tropical Diseases, 21, 9.

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