Mkn 7

Human gastric mucosa cell line (GES-1) used in this study was procured from BeNa Culture Collection (Beijing, China). Human GC cell lines (HGC-27, AGS) were bought from Cell Bank of the Chinese Academy of Sciences (Shanghai, China); both MKN-7 and MKN-45 cell lines were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China). RPMI-1640 commercially acquired from Thermo Fisher Scientific (Waltham, MA) was used to culture GES-1, HGC-27, MKN-7, and MKN-45 cells under 37°C and 5% CO₂. AGS cells were cultivated in F12K medium (Gibco). In addition, 10% fetal bovine serum (FBS; Gibco) and 1% penicillin-streptomycin (Gibco) both served as medium supplements. SF1670 (1 μM), an inhibitor of PTEN (phosphatase and tensin homolog), was purchased from MedChemExpress (South Brunswick, NJ).

GES-1 (CL-0563) and GC cell line MKN-7 (CL-0574) were purchased from Procell (Wuhan, Hubei, China). GC cells AGS (CRL-1739), SNU-16 (CRL-5974), NCI-N87 (CRL-5822) were from ATCC (Manassas, VA, USA). All cells were cultivated at 37 °C in Roswell Park Memorial Institute-1640 complete medium (PM150110B, Procell) plus 10% FBS with 1% penicillin/streptomycin at 5% CO₂.
The overexpressed (oe) DNA plasmids oe-MYH11, oe-TNFRSF14, oe-DNMT3B and control oe-negative control (NC) used for cell transfection were from VectorBuilder (Guangzhou, Guangdong, China). Lipofectamine 2000 (Thermo Fisher) was used for all transfections. Dimethylsulfoxide (DMSO) and DNA methylation inhibitor 5-aZa-CDR (CAS No. 2353-33-5) were purchased from Sigma-Aldrich Chemical Company (St Louis, MO, USA). The GC cells were treated with 5-aZa-CDR dissolved in DMSO at 5 μM for 24 h for DNA demethylation [15 (link)].