Cd86 pe cy7 clone gl 1

Manufactured by BioLegend

CD86 PE-Cy7 (clone GL-1) is a fluorochrome-conjugated antibody that binds to the CD86 cell surface molecule. CD86 is a co-stimulatory molecule expressed on antigen-presenting cells and is involved in T cell activation.

Automatically generated - may contain errors

Lab products found in correlation

Cd11c brilliantviolet 650 clone n418, by BioLegend (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions) B220 apc clone ra3 6b2, by BD (1 mentions) Cd11b apc clone m1 70, by BioLegend (1 mentions) Cd4 apc cy7 clone gk1, by BioLegend (1 mentions) Facs lsr 2, by BD (1 mentions) Facsaria cell sorter, by BD (1 mentions) Efluor 780, by Thermo Fisher Scientific (1 mentions) Dylight 594 conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Macsquant analyzer, by Miltenyi Biotec (1 mentions) Anti cd16 32antibody, by BioXCell (1 mentions) Efluor 780, by BD (1 mentions)

Spelling variants of this product

CD86-PE (65 citations) CD86-PE-Cy7 (23 citations) CD86 (GL-1, (16 citations) CD86 (clone GL-1, (9 citations)

3 protocols using cd86 pe cy7 clone gl 1

Evaluating Transduced Dendritic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

In addition to determining the downregulation of expression of the target gene, the efficacy of transduction was assessed by flow cytometry as the percentage of enhanced green fluorescent protein-positive (EGFP⁺) cells. The surface phenotype characteristics of transduced DCs were also evaluated by flow cytometry. For this purpose, cells were stained with anti-MHC II APC-Cy7 (clone M5/114.15.2; BioLegend), anti-CD40 BrilliantViolet 605 (clone 3/23; BD Pharmingen), CD80 PerCP-Cy5.5 (clone 16-10A1; BioLegend), CD86 PE-Cy7 (clone GL-1; BioLegend) and CD11c BrilliantViolet 650 (clone N418, BioLegend) monoclonal antibodies and elimination of the dead cells was carried out by using the DAPI dye.

Szczygieł A., Węgierek-Ciura K., Wróblewska A., Mierzejewska J., Rossowska J., Szermer-Olearnik B., Świtalska M., Anger-Góra N., Goszczyński T.M, & Pajtasz-Piasecka E. (2023). Combined therapy with methotrexate nanoconjugate and dendritic cells with downregulated IL-10R expression modulates the tumor microenvironment and enhances the systemic anti-tumor immune response in MC38 murine colon carcinoma. Frontiers in Immunology, 14, 1155377.

+ Open protocol

+ Expand

Flow Cytometry Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

Viability dye (cat. L34968) and CFSE (cat. 65-0850-84) were both purchased from Invitrogen. The following monoclonal antibodies (mAbs) were also purchased from Invitrogen: H-2Kd APC (clone SF1-1.1.1, cat. 17-5957-82), CD8a PE-Cy7 (clone 53-6.7, cat. 25-0081-82), MHCII Pacific Blue (clone M5/114.15.2, cat. 48-5321-8), and CD11c PE-Cy7 (clone N418, cat. 12-7177-82). The following mAbs were purchased from Biolegend: CD4 APC/cy7 (clone GK1.5, cat. 100414), CD11b APC (clone M1/70, cat. 101212), CD86 PECy7 (clone GL-1, cat. 105014). B220 APC (clone RA3-6B2) was purchased from BD Pharmingen (cat. 553092). We obtained anti-CD5.1 (Ly5.1)-FITC, clone H11-86.1, from BD Pharmingen (cat 557487).

Heja D., Zhao D., Cody E., Cumpelik A., Lim P.C., Prado-Acosta M., Palma L., Dellepiane S., Chun N., Ferrara J, & Heeger P.S. (2022). Mannan binding lectin promotes murine graft versus host disease by amplifying lipopolysaccharide-initiated inflammation. Transplantation and cellular therapy, 28(8), 472.e1-472.e11.

+ Open protocol

+ Expand

Flow Cytometric Analysis of Germinal Center B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Spleens were prepared into single cell suspension and red blood cells were lysed
in cold distilled water. After filtered through 70-µm nylon mesh and counting
using a MACSQuant analyzer (Miltenyi Biotec), cells were incubated with anti-CD16/32
antibody (Bio X Cell) to block Fc receptors and stained with fixable viability dye eFluor
780 (eBioscience). Cells were then stained for 20 min in staining media (Hank’s
balanced salt solution, 3% FBS, 0.02% sodium azide, 1 mM EDTA) with
primary antibodies including B220-FITC, B220-eVolve 655 (clone RA3–6B2),
GL7-eFluor 660, GL7-eFluor 450 (clone GL7), CD95-PE, CD95-APC (clone 15A7) (eBioscience),
peanut agglutinin (PNA)-biotin (Vector Laboratories), CD86-Pe-Cy7 (clone GL-1; BioLegend)
or CD184-biotin (2b11/CXCR4; BD Biosciences). Cells stained with biotin-labeled antibodies
were incubated with streptavidin-eFluor 450 (eBioscience). For intracellular staining,
cells were stained with fixable viability dye eFluor 780, permeabilized and fixed using a
cytofix/cytoperm plus kit (BD Biosciences) according to manufacturer suggested protocol.
Cells were then stained with YY1 antibody (H-414; Santa Cruz Biotechnology) and DyLight
594-conjugated secondary antibody (Jackson ImmunoResearch). Flow cytometry analysis was
performed on an LSRII FACS or a FACSAria cell sorter (BD Biosciences), and analyzed using
FlowJo software (FlowJo).

Trabucco S.E., Gerstein R.M, & Zhang H. (2016). YY1 regulates the germinal center reaction by inhibiting apoptosis. Journal of immunology (Baltimore, Md. : 1950), 197(5), 1699-1707.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!