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Sc 9447

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

Sc-9447 is a laboratory product offered by Santa Cruz Biotechnology. It is designed for use in scientific research applications. The core function of this product is to [description not available].

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Lab products found in correlation

Ab17721, by Abcam (2 mentions) Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (2 mentions) Ab23980, by Abcam (2 mentions) Ab18465, by Abcam (2 mentions) Ab12169, by Abcam (2 mentions) Sc 6217, by Santa Cruz Biotechnology (2 mentions) Sc 7899, by Santa Cruz Biotechnology (1 mentions) Ab290, by Abcam (1 mentions)

3 protocols using sc 9447

1

Antibodies for Protein-Protein Interactions

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The antibodies used are as follows: rabbit αGFP: ab‐290, Abcam; rabbit αCDK2AP1: in‐house generated, V41 [24]; rabbit αHDAC1: H51, SC‐7872, Santa Cruz; rabbit αHDAC2: SC‐7899, Santa Cruz; goat αMTA2: SC‐9447, Santa Cruz Biotechnologies, Inc., Dallas, Texas, USA; goat αMBD2: EB07538, Everest Biotech, Bicester, England, UK; rabbit αCHD4/Mi2beta: A301‐081A, Bethyl Laboratories, Inc., Montgomery, Texas, USA; rabbit αMTA1: A300‐911A, Bethyl; goat αRbAp46: SC‐8272, Santa Cruz; and rabbit αMBD3: A302‐528A, Bethyl.
Spruijt C.G., Gräwe C., Kleinendorst S.C., Baltissen M.P, & Vermeulen M. (2020). Cross‐linking mass spectrometry reveals the structural topology of peripheral NuRD subunits relative to the core complex. The Febs Journal, 288(10), 3231-3245.
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2

Protein Interactome Analysis of Bcl11b in Scid.adh.2c2 Cells

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Protein extracts from Myc-Flag-tagged Bcl11b-infected Scid.adh.2c2 cells were subjected to immunoprecipitation as described previously43 (link). Nuclear extracts were prepared using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific). The antibodies used for the immunoblot analyses were anti-Chd4 (A301–081A, Bethyl), anti-Mta2 (sc-9447, Santa Cruz Biotechnology, Inc.), anti-HDAC2 (ab12169, Abcam), anti-Rest (12C11–1B11, Caltech Protein Expression Center), anti-Ring1b (A302–869A, Bethyl), anti-LSD1 (ab17721, Abcam), anti-Runx1 (ab23980, Abcam), anti-Bcl11b (ab18465, Abcam), anti-Lamin B (sc-6217, Santa Cruz Biotechnology, Inc.), and anti-Myc (My3, MBL).
Hosokawa H., Romero-Wolf M., Yui M.A., Ungerbäck J., Quiloan M.L., Matsumoto M., Nakayama K.I., Tanaka T, & Rothenberg E.V. (2018). Bcl11b sets pro-T cell fate by site-specific cofactor recruitment and by repressing Id2 and Zbtb16. Nature immunology, 19(12), 1427-1440.
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3

Protein Interactome Analysis of Bcl11b in Scid.adh.2c2 Cells

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Protein extracts from Myc-Flag-tagged Bcl11b-infected Scid.adh.2c2 cells were subjected to immunoprecipitation as described previously43 (link). Nuclear extracts were prepared using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific). The antibodies used for the immunoblot analyses were anti-Chd4 (A301–081A, Bethyl), anti-Mta2 (sc-9447, Santa Cruz Biotechnology, Inc.), anti-HDAC2 (ab12169, Abcam), anti-Rest (12C11–1B11, Caltech Protein Expression Center), anti-Ring1b (A302–869A, Bethyl), anti-LSD1 (ab17721, Abcam), anti-Runx1 (ab23980, Abcam), anti-Bcl11b (ab18465, Abcam), anti-Lamin B (sc-6217, Santa Cruz Biotechnology, Inc.), and anti-Myc (My3, MBL).
Hosokawa H., Romero-Wolf M., Yui M.A., Ungerbäck J., Quiloan M.L., Matsumoto M., Nakayama K.I., Tanaka T, & Rothenberg E.V. (2018). Bcl11b sets pro-T cell fate by site-specific cofactor recruitment and by repressing Id2 and Zbtb16. Nature immunology, 19(12), 1427-1440.
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