Wild type c57bl 6n mice

Manufactured by Taconic Biosciences

Sourced in Denmark

Wild-type C57BL/6N mice are a commonly used inbred mouse strain. They serve as a standard control strain in a variety of research applications.

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Lab products found in correlation

Fvb n mice, by Charles River Laboratories (1 mentions) R m h, by Ssniff (1 mentions) D12492, by Research Diets (1 mentions)

Close spelling variants of this product

C57BL/6N mice Wild-type mice C57BL/6N

5 protocols using wild type c57bl 6n mice

C57BL/6N Mouse Protocol

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Wild-type C57BL/6N mice (4 to 6 wk old) were purchased from Taconic Biosciences. All animal procedures were performed according to NIH guidelines and approved by the Committee on Animal Care at Massachusetts General Hospital and Harvard University.

Lion M., Muhire B., Namiki Y., Tolstorukov M.Y, & Oettinger M.A. (2020). Alterations in chromatin at antigen receptor loci define lineage progression during B lymphopoiesis. Proceedings of the National Academy of Sciences of the United States of America, 117(10), 5453-5462.

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Genetically Modified Mouse Models for Immune Research

Cited 1 time

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Ifnar1^−/− (Line 314), Ccr7^−/− (Line 8453), and LysM-eGFP (Line 342) were obtained from the National Institute of Allergy and Infectious Diseases intramural research repository at Taconic Farms. Wild-type C57BL/6N mice were obtained from Taconic Farms. Ifnar1^fl/fl mice (B6(Cg)-Ifnar1tm1.1Ees/J; #28256) were obtained from Jackson Laboratories. CD169-cre mice have been previously reported.^{68 (link)} Embryos were obtained from Riken, rederived and bred in-house to generate CD169-cre Ifnar1^fl/fl conditional knockout (cKO) mice. hStat2-KI mice in a C57BL/6 background have been reported previously and were bred at Washington University School of Medicine.^{10 (link)} Male and female mice from 6–12 weeks of age were used for experiments. Mice were housed under specific pathogen-free conditions and provided standard rodent chow and sterile water as necessary. All animal studies were approved by and performed in accordance with the Animal Care and Use Committee of the National Institute of Allergy and Infectious Diseases or Washington University School of Medicine.

Benten W.P., Lieberherr M., Stamm O., Wrehlke C., Guo Z, & Wunderlich F. (1999). Testosterone Signaling through Internalizable Surface Receptors in Androgen Receptor-free Macrophages. Molecular Biology of the Cell, 10(10), 3113-3123.

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Experimental Protocols for AMCase-Deficient Mice

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The National Institute of Allergy and Infectious Diseases Division of Intramural Research Animal Care and Use Program, as part of the National Institutes of Health Intramural Research Program, approved all of the experimental procedures (protocol “LPD 16E”). The Program complies with all applicable provisions of the Animal Welfare Act (http://www.aphis.usda.gov/animal_welfare/downloads/awa/awa.pdf) and other federal statutes and regulations relating to animals. AMCase-deficient mice on a C57BL/6N background were kindly provided by L. Fitz and Pfizer. Wild-type C57BL/6N mice, and IL-13-deficient and TCRα-deficient mice on a C57BL/6N background, were obtained from Taconic Farms Inc. Male and female mice between the ages of 6 weeks and 12 weeks were used randomly to begin experimental models because of limited availability, and no sex-specific differences were observed. Groups in individual experiments were sex-matched and age-matched. All animals were housed under specific-pathogen-free conditions at the National Institutes of Health in an American Association for the Accreditation of Laboratory Animal Care–approved facility.

Vannella K.M., Ramalingam T.R., Hart K.M., de Queiroz Prado R., Sciurba J., Barron L., Borthwick L.A., Smith A.D., Mentink-Kane M., White S., Thompson R.W., Cheever A.W., Bock K., Moore I., Fitz L.J., Urban JF J.r, & Wynn T.A. (2016). Acidic chitinase primes the protective immune response to gastrointestinal nematodes. Nature immunology, 17(5), 538-544.

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Transgenic Mouse Model Experiments

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For the imaging experiments
female C57BL/6N wild-type mice (Taconic, Skensved, Denmark) and female
homozygous Abcb1a/1b^(−/−) and heterozygous Abcb1a/1b^(+/−) mice backcrossed to the C57BL/6 background (N >10) were used. For the other experiments female wild-type FVB/N mice
(Charles River, Sulzfeld, Germany) and female Abcb1a/1b^(−/−) mice with an FVB genetic background
(Taconic, Germantown, NY, USA) were used. All animals were housed
in groups under individual ventilated cage conditions in polysulfon
type III cages. The environmental conditions were as follows: temperature,
22 ± 3 °C; humidity, 40% to 70%; and a 12-h light/dark cycle
(lights on at 6:00) with free access to standard laboratory rodent
diet (ssniff R/M-H, ssniff Spezialdiäten GmbH, Soest, Germany)
and tap water. An acclimatization period of at least 1 week was allowed
before the animals were used in the experiments. All animal experiments
were approved by the national authorities (Amt der Niederösterreichischen
Landesregierung), and all study procedures were performed in accordance
with the European Communities Council Directive of September 22, 2010
(2010/63/EU). All efforts were made to comply with the 3Rs principle
in this study.

Wanek T., Römermann K., Mairinger S., Stanek J., Sauberer M., Filip T., Traxl A., Kuntner C., Pahnke J., Bauer F., Erker T., Löscher W., Müller M, & Langer O. (2015). Factors Governing P-Glycoprotein-Mediated Drug–Drug Interactions at the Blood–Brain Barrier Measured with Positron Emission Tomography. Molecular Pharmaceutics, 12(9), 3214-3225.

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C57BL/6N Mice Pharmacological Interventions

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C57BL/6N wild-type mice for experiments were obtained from Taconic. UCP1-Cre mice and B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J (mTmG) mice are from Jackson Laboratory. The Ucp1-cre mice were crossed with mTmG mice to remove the flox-flanked resistance cassette. Mice were housed at 23°C in a 12 hr light/dark cycle with free access to normal chow. All experiments used matched littermates. Experimental procedures were approved by the Boston University Institutional Animal Care and Use Committee. For diet studies, 8-week-old C57BL/6N wild-type male mice were fed a diet with 60% kcal% fat (high fat diet, Research Diets, D12492) for 9 weeks. During the last 6 weeks of the diet, the mice were intraperitoneally (i.p.) injected daily with rosiglitazone (10 mg/kg), or roscovitine (50 mg/kg), or CL-316,243 (1 mg/kg), or vehicle. For cold exposure experiment, body temperatures were measured rectally using a digital thermometer.

Wang H., Liu L., Lin J.Z., Aprahamian T.R, & Farmer S.R. (2016). Browning of White Adipose Tissue with Roscovitine Induces a Distinct Population of UCP1+ Adipocytes. Cell metabolism, 24(6), 835-847.

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